Initial Study On Cell Culture Of Gynostemma Pentaphyllum (Thunb.) Makino

The cell culture was studied in Gynostemma pentaphyllum (Thunb.) Makino .This paper reports the induction and screen of callus. And it also explains the callus growth ^ some physiological and biochemical characters and the secondary production accumulation under the different conditions.The results were as follows:1. By experiments ,the optimal medium was chosen for the callus induction and growth of Gynostemma pentaphyllum . It is 65 with the addition of 2.0mg/L 2,4-D and 0.5mg/LKT.2. The effects on the callus growth by the different factors:(1) In the white light and the dark ,the callus growth were for about 27 days ,and the growth circle curve was like "S". It could be divided into lag phases exponential growth phase and stationary phase. The cell grew more rapidly in the white light than in the dark.(2) In the solid culture, the inoculum quantity of 3g.FW/50ml medium was best; The optimal concentration of Surose was 30g/I,and the one of Glucose was 40g/L;As the sources of carbon, the effectiveness of Glucose was better than Surose on the cell growth; The 0.05% Agarose was best to the cell growth.3. In the white light and the dark, the contents of soluble protein, the activities of peroxidase and superoxide dismutase, all showed two peaks on the different days; the soluble protein content was higher in the white light.4. The secondary production of the callus was accumulated primarily in stationary phase. In the white light and the dark, the G.saponium contents were almost the same level; but the flavoloids content was higher in the former condition.In the cases of the 3g.FW/50ml medium inoculum quantity ,the 30g/LSucrose or the 40g/U Glucose and the 0.1% Agarose, the secondary products contents reached the highest level respectively.