Studies On Pathology And Immunological Status Of Experimental Avian Myelcytomatosis

Two groups of 1-day-old meat-type chickens were inoculated with NM8761 and NM9996 strain of ALV-J respectively, and control group was inoculated with nothing. Enzyme linked immunosorbent assay(ELISA) test, Lymphokine abstraction test, ANAE stain were used for observation of humoralimmunity and cell-mediated immunity, and polymerse chain reaction(PCR) was used for detecting virus. In the meantime, all tissue samples were removed and fixed in 10% formalin, then embedded in paraffin, sectioned at 5um.and stained with hematoxylin and eosin(H.E), and examined for microscopic lesions.Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.