Studies On Inactive Mechanism Of Sodium Dichloroisocyanurate On Bacillus Thuringiensis And Compound Disinfectant

si\kwoTm(Bombyx mori) bacterial toxicosis was caused by Bacillus thuringiensis (B.t) when silkworm ate enough amount of its spore and parasporial crystal .It was an acute toxicosis and its pathogeny was Bacillus thuringiensis .It lied in all kinds of soil and air. B.t had strong insectcidal ability ,and it could kill a lot of creatures of Arthropoda, Protozoa, Nemathelminthes and Platyhelminthes.B.t was the most comprehensive microorganism being used in biotic pesticide.With the more comprehensive use of BT biotic pesticidal, silkworm bacterial toxicosis threntened the development and safety of sericulture in our country.For these reasons, it had great significance developing new medicine with steady character and excellent disinfectant effect to B.t to prevent silkworm from infecting this disease.And the study of disinfectant mechanism of chlorine preparation to parasporal crystal and spore of Bacillus thuringiensis could provide practice with academic direction.We studied these disinfectant mechanisms of sodium dichloroisocyanurate to parasporal crystal and spore by biological assay,electron microscope technique,electrophoresis technique and so on.The major achievements obtained in these experiments were as follows.1. The result of SDS-PAGE indicated that the pedigrees of the treated parasporal crystal had changed obviously and chlorine preparation distroyed primary structure of parasporal crystal and made it lose its activity.2. The result of SEM showed that sodium dichloroisocyanurate damaged the shape structure of parasporal crystal, and then holes appeared on its surface.If treatment time was long enough,the parasporal crystal would lose its former41appearance and became loose corps,that is,its stereoscopic structure was damaged.The damage of primary and stereoscopic structure of parasporal crystal made its virulence receded and even lost completely.3. chlorine preparation could destroy external structure of spore. And the fabric of spore was no longer compact,thus its ability of protection was lower.4. chlorine preparation could destroy inner structure of spore. The medicine penetrating into spore damaged inner tissue.When the damage was serious enough,the germination ability of spore was lower and even lost completely.5. We developed a compound disinfectant ,named XDJ-III, using sodium dichloroisocyanurate as main medicament which had high stability and security and some substance as accessorial medicament which could increase effect,postpone erosion, increase penetration ability and pervasion ability. XDJ-III could disinfect silkworm rooms and tools .leaves surface when being confected to a certain concentration solution. It had good disinfecting effect on silkworm's main pathogens.At the same time,it could be used as smoking medicine disinfecting silkworm rooms.Being used as smoking medicine, XDJ- El had many strongpoints.For example,it could give out smoke rapidly and had no eyeable fire.lt could loose its active ingredient more than 99.8% and the leftover was very little.6. Being used as disinfectant of silkworm rooms and tools, the available chlorine concentration of XDJ-III should be 0.5%. As disinfecting leaves, the available chlorine concentration of XDJ-III should be 0.3%. We'd better use up the solution, otherwise it would decrease its effect. Being used as smoking disinfectant, its effective concentration was about 5~8g/m3,and we must close windows and doors more than 12 hours. And when disinfecting silkworm and rearing bed, its concentration was about 1.5g/m3 and 30 minutes later we should air the silkworm room. It did not have bad influence on silkworm growth and development.7. XDJ-III had obvious effect of postponing erosion of metal. The manufacture technics , preservasion and use were very simple and easy .It could meet the need of multiuse medicine?in sericulture.