| Host leading diseases are severe challenges which will be faced by Chinese forestry development in the 21st century. This paper studied control factors and their compound treatment which could improve microecological environment in the root system and rhizosphere soil in order to prevent the occurance of poplar canker disease and supply theorial and practical basis for this kind of host leading diseases. This paper was composed by two parts:The first part studied the optimizing of the factors on controlling the microecological environment in the root system and rhizosphere soil:First, optimize the culture conditions of ECM fungi. The result suggested MOO land M002 was suitable to grow in the culture medium of MMN and PDA which were sterilized for ten minutes; the suitable temperature and pH scopes were 25~30癈 and 4-9; the suitable carbon and nitrogen sources were glucose, starch and beef extract. The ferment condition of M001 includes 30g/L glucose, 15 g/L beef extract, 5% the inoculation quantity and 1.5 L/L/m ventilation quantity.Second, the resistance to the poplar canker disease answered to inoculating ECM fungi. Each inoculum set up three levels (10,25,50g/plant), the control was 25g/plant medium. The result suggested the cutting stock per plant inoculated M001 25g could promote its growth, improve its survival rate, relative turgidity and strengthen the activity of peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase.Third, the poplar root system development answered to using the growth regulators. NI and I] set up four levels(50,100.200, SOOppm), according to weight proportion NI: I] set up 1:1, 3:2, 4:3.the mixture set up the same four levels. The cutting stock soaked in the first three levels solution for one hour, the fourth for 5 second, the control was water, GI and A) lOOppm. The result suggested NI lOOppm treatment could significantly promote the poplar cutting stock height .diameter at soil line and lateral root growth.Fourth, the effect of the growth regulator on the ECM fungi. In the first treatment added 65%(according to weight proportion) NI 100 ppm in the culture medium. The control added the same proportion water. The result suggested the growth regulator had no effect on theECM fungi.Fifth, the biomass of poplar cutting stock answered to using superabsorbent. SA and SB used the method of mixing with the soil and soaking the root, set up 0.5%, 1% and 2% levels; SC used the method of mixing with the soil, set up 7.5%, 15% and 30%, the control had no superabsorbent. The result suggested SC15% treatment could improve the survival rate and biomass of poplar cutting stock.The second part studied the effects of the compound control factors on the resistance to poplar canker disease.When the compound factors were used in poplar cutting stock, the experiment set up five treats:(1)mixed 125g M001 inoculum, saturant made by 10g SC and soil (according to volume proportion, saturant : soil =15%)together. put the cutting stock which was soaked by G into the mixture; (2)mixed the saturant which had absorbed the GI, 125g M001 inoculum and soil together; (3)mixed the same quantity inoculum medium and soil, no superabsorbent, put the cutting stock soaked in the water into the mixture;(4) and (5) treatments used the same method with (1) and (2), but the growth regulator were A 100ppm. The result suggested that (4) treat could promote the growth, improve growth potential, increase the content of chlorophyll a, b and relative turgidity, strengthen the activity of peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase.When the compound factors used for Poplar X beijingensis W.Y. Hsu in field afforestation, the experiment adopted the orthogonal test, P. Xbeijingensis used the method of mixing with the soil (the compound : soil = 1:1000) and the method of soaking the root, the control had no the compound preparation. The result suggested that the 8th treat in the method of mixing with the soil took the best effect on the P. Xbeijingensis... |
