Optimization Of Tissue Culture System And Anti-fungal Transfection Of Xinjiang Muskmelon

Fungal disease has been a serious threat to production and trait of Xinjiang muskmelon in recent years. We attempt to transfer Xinjiang muskmelon via Agrobacterium tumefaciens carrying chitinase and β -1,3-glucanase in order to enhance the abilities of anti-fungal.In order to establish the transgenic plant system, the cotyledons of muskmelon as explants, regenerated plantlets were used in culture. Shoots regeneration were induced on basic medium MS medium supplemented with 0-2.5mg/L 6-BA.The highest frequency shoot regeneration observed is 89%. The induction frequency of shoot of melon varieties is depended on different concentration of hormones in the medium. To achieve the high rate of rooting, the concentration of hormone 6-BA was gradually decreased during the whole growth period. The 1/2 MS medium with 0.5mg/L IBA and NAA was used for this propose.On the basis of regeneration protocol of Xinjiang muskmelon, two genes were transfected into muskmelon plant and selected of kanamycin in the medium. After kanamycin resistant plants were obtained, transgenic plants were determined by PCR and confirmed by Southern dot blotting. The expression of chitinase was observed by using SDS-PAGE analysis.