| Three experiments were conducted to study the parameters of producing feed dicalcium phosphates from the residue of inositol industry and to evaluate the relative biological availability of the product using chicks. Growth rate , bone ash of tibias, plasma calcium, plasma phosphorus and plasma alkaline were measured during the biological evaluation. A farm level experiment was conducted with layers chicks to examine the feeding of the product.A orthogonal experiment was conduct to select the suitable acidify and neutralization condition during making feed level dicalcium phosphates form inositol residue. Using treatment condition selected from this experiment, we get a product that is homogeneity in particle size, white in color, and contains 16% of phosphates and 26.8% of calcium. This product reach the national criterion of feed level dicalcium phosphates (GB8258-87).One hundred and sixty AA chicks were used to determine the relative biological availability of the product in the second experiment. The chicks were divided into two groups. The diets of group A was supplement with dicalcium phosphate made from inositol residue, while that of group B was supplemented with pure calcium phosphate. Phosphates were added to the corn-soybean basal diet to provide 0.53%, 0.60%, 0.68%, 0.75% and 0.90% phosphorus (dietary non-phytate phosphorus level is 0. 30%, 0. 37%, 0. 45%, 0. 52% and 0.67% accordingly). The calcium level was 0. 91%for all diets. Right tibias were gotten for measuring bone ash, and plasma for measuring plasma calcium, plasma phosphorus and plasma alkaline. The results indicated, the dietary non-phytate phosphorus level did not affect the weight gain (p>0. 05), the weight gain was higher at 0. 37%, 0. 45%, 0. 52% dietary non-phytate phosphorus level than that at 0.30% (p<0. 05), and the 0.37% dietary non-phytate phosphorus level had higher weight gain than 0. 67%( p<0. 05). The weight gain at 0. 37% dietary non-phytate phosphorus level was the highest, and that at 0. 30%.dietary non-phytate phosphorus level was the lowest in two groups. Bone ash of the tibias increased with the increasing dietary non-phytate phosphorus level in total, and bone ash at 0. 30% dietary non-phytate phosphorus level was the lowest, while bone ash at 0. 67% dietary non-phytate phosphorus level was the highest. In group A, bone ash at 0. 30% dietary non-phytate phosphorus level was lower than the other, bone ash at 0. 37%, 0. 67% dietary non-phytate phosphorus level was better than at 0. 30%, 0. 45%, 0. 52% level in group B (p<0. 05). Plasma alkaline decreased when dietary phosphorus increased in total. Plasma calcium was the highest when the dietary phosphorus level was 0.30%, the lowest when that was 0. 37% . In group A the dietary had no significant influence on plasma calcium, and plasma calcium at 0. 30% dietary non-phytate phosphorus level was better than at 0. 37%, 0. 45%, 0.52% level (p<0. 05) . In group A, plasma phosphorus at 0. 30% dietary non-phytate phosphorus level was less than the other (p<0. 05). In group B, plasma phosphorus at 0.67% dietary non-phytate phosphorus level was better than 0.30%, 0. 37% level(p<0.05), and plasma phosphorus increased with dietary phosphorus level increasing in two groups. The relative biological value was calculated based on body weight , bone ash , plasma calcium and plasma phosphorus, which were 96%, 93%, 92% and 104% respectively, the average value was 96.25%.One hundred and eighty Romance chickens were used to check the effect of the dicalcium phosphates by using randomized complete block design in experiment HI. The result showed that the total egg weight, average egg weight, feed: intake ratio and tender or broken eggs ratio have no significant between two treatments (p>0. 05), Egg thicker on 15d was higher than control group (p<0.05). There were no significant difference (p>0. 05) in egg strength and thicker on 30d, egg strength on 15d among different treatment. |
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