Study On Valuation Of Pumpkin Virus In Heilongjiang Province And Selection Of Resistant Pumpkin Resources

Pumpkin virus di sease is popu1ar in Ilei InngJi ang provj nce in the year of hightemperature and drought, especial1y serious in the west area. The typi ca1 symptomj s fold and b1 istering, even d istortj on, 1ops i ded j ] ] --] eaf, shorti ng p] ant\, thereare motley with 1ight green and deep green, and some of the mot]ey areye1wt MM 1eave M tny: the i].1--1ed has arias mosai c, 1ops i ded, and thetissue of the i11--1eaf has deteriorated exceeding1y, with s1ender pteridophyte1.eaves, i l1 p1ant is green--yeI 1ow, obvious1y shortened in joint, the who1e p1antserious1y shortened and grows thickly.Inspected and Surveyed in the method of sero1ogic 49. 2%samples are WMV--2,19. 8% saJnp1es are SqMV 12. 5% samp1es are CMV, in 183 Samp1es co11eted from severalareas of HLJ province, and the ratio of three viruses is different in differentarea, HarBin area: 53. 3% samp1es are WMV--2, 20. 4% sanp1es are SqM'V 16. 7% samp1esare CMV, QiQiHar area: 47. 8% samp1es are WMV2, 32. 2% saJnples are SqMV 10. 4%samP1es are CMV, MuDanJiang area: 52. 5% samp1es are mp--2, 13. 3% saJnp1es are SqMV12. 5% sanp1es are CMV, JiaMuSi area:43. 2% sanp1es are op2, l3. 3% samp1es areSqMV, 10. 4% samp1es are CMV. The ratio of WMV--2 is 43. 2%ed3. 2% in every area. Itis obvious that different areas has the same main virus resource:WMV--2.The samp1e of typica1 sWhptom which was separated in sing1e spot, propagatingin Cucufoita pap 'host' can infect Cucumjs satfwS, CjtTh11us wharS, C?ucumfsme1O, for gOUrd C qU1bOa Phaseo1us rv1gartS cannot infect P1sum sati mp N g1utiDoS4 LNoPersicum escofentum DUthe sboniU4 A/jcotiano tabacu4 The lengh ofstring virus partic1es which were observed through e1ectronic microscope is600--750nm. Ihathy p1ant 1are dsch wr ed in mp1ight section, ch1oroplastmembrane was comp1ete and c1ear, basica1 partica1 pieces and basica1 materi a1Piare wr pet in forfot edIn ]eres innd by ew he ch]det thediso1ved boital wtital nd boital lwtiaI piare m disinwi theica] ghic1es wereindistinct the pet in ditw;Wt we11ed ed bo m a tw qUantity toitresswiiCh fortw the --t mp1ar stnJcthe be and he ew1t Of nsisthe Of ootSidetw is: rot is the bo the miVdl tbe outeids bo is 36 fors, the lbotation Ofdiluting is 10tThe research of the se1ection for mp--2expressed that being inocu1ated incoty1edon time or 1true 1eaf time, the most suitab1e temperature is 25t, and the2most suitable consistency is lOOg ill-leaf juice diluted 2 times.Using the result of this study to identify the disease resistance in seed! ing time of 55 pumpkin materials which were inoculated with WMV-2. The result is:33 materials are S, 17 materials are MR, 5 materials are R. This result provides test materials for disease resistance breeding.Surveying and de terming the conductirity, chlorophy content and PPO. activity of pumpkin materials after being inoculated 3.6.9.12.15.18.21.24.27 days. The result is:the change of conductirity, chlorophy content and PPO activity of healthy plant was small, however the inoculated plant, conductirity of relativity attained largest number after inoculated 21 days then decreased;chlorophy content decreased and PPO activity attained largest number after inoculated 21 days then decreased.