The Use Of Microsatellite And RAPD Markers For Prediction Of Heterosis In Lingnan Yellow Chicken

The genetic variation , heterozygosity ,band sharing rate and index of genetic polymorphism of 3 chicken breeds (or lines) of Lingnan Yellow Chicken were analyzed by using 10 pairs of microsatellite primer and 20 RAPD primers in this study. It was performed that correlation analysis between genetic distance of two parents and weight heterosis or weight heterosis rate of F, from KXF and K X A chicken populations by applying the two kinds of molecular markers respectively. Microsatellite alleles related to heterosis or to heterosis rate were detected.The results indicated that 7 of all the 10 microsatellite markers were much more polymorphic than the other 3 markers. Genetic variations of 9 polymorphic loci in chicken genome were detected. The average number of alleles detected by a primer pair were 4.2 and the average values of polymorphism information content was 0.44. The 13 RAPD markers of the 20 RAPD markers could produce reproducible results. 117 loci's variations in chicken genome were detected by the 13 markers. The number of the average reproducible major bands produced by a RAPD primer were 9 including 5.4 polymorphic bands.The genetic distance between parents based on the microsatellite and on RAPD are different and they only have little correlation .The heredity similarity of the three lines based on microsatellite and on RAPD are not the same. Which one is correct relies on further study. The correlation between the distance and heterosis is small, positive or negative. The microsatellite distance between parents in KXF is markedly correlated with the weight heterosis in eight week and the RAPD distance of parents in KXF is markedly positive correlated with weight heterosis in naught week. Special band is detected in line F by using random prime R03 and in line A using random prime R12.The frequence of all bands is different in all three lines.Allele 267bp detected by Microsatellite primer MCW0073 and 124bp by mcw0148 arepositive correlated with weight heterosis (rate)in naught week. Alleles 250bp,230bp,215bp detected by MCW0073 and 111bp and 89bp by MCW0148 are negative correlated with weight heterosis in naught week. Allele 118bp detected by mcw0148 is possible positive correlated with weight heterosis in naught week.It is necessary to using more prime to detect more locus to achieve more imformation .It is a primary work to try to find the markers which are linked with the quantitative trait locus and the markers which are polymorphic and repeatable in heterosis prediction.The possibility of heterosis prediction using DNA polymorphic markers is difficult to answer because the correlation between the heterozygosity and heterosis is largely different. Thus, to predict heterosis using molecular markers relies on the study of heredity of heterosis.