Studies On The Folliclar Oocytes In Vitro Maturation And Cryopreservation In Porcine

In this paper, porcine ovaries collected from slaughters were used. Factors affecting oocytes IVM, such as addition of hormones, different type of serums, follicular fluid and size of follicle were studied. Cryopreservation of oocytes in different growth phase was also studied in this paper. The results were as follows:1. Addition of PMSG (10 IU/ml) and hCG (10 IU/ml) in the maturation medium had significantly effected on the maturation rate of IVM, the maturation rate of experimental group was very significant higer than control group (P<0.01).2. The maturation rate of IVM was very significantly higher in the culture medium contain 10%ECS (72.86%) than 10%NCS (62.21%) (P<0.05).3. Addition of 10% porcine follicular fluid (pFF) to mature medium inhabits the in vitro maturation of oocytes. The maturation rate in medium without pFF (79.60%) was significant higher than medium contain 10% pFF (68.51%) (P<0.05).4. Oocytes from different size of follicular had different development ability. Oocytes collected from big ( >5mm) and medium ( 2 ~ 5mm) follicles had a higher maturation rate than oocytes from little (<2mm) follicles (P<0.05).5. When oocytes maturation for 48h in vitro, removal of hormone supplements from mature media at 24h after culture enhance oocytes maturation and cumulus expansion. And the maturation rate (83.25%) was very significant higher than oocytes that culture for 24hin the media without hormone subsequent added hormone for another 24h (63.94%) (P<0.01). also higher than oocytes culture for 48h in media without hormones (60.01%) (P<0.05).6. The experiment was studied the cryopreservation of porcine oocytes in different growth stages. The results showed that oocytes in MII stage had higher antifreeze ability than GV stage and oocytes culture for 24h. The survival rate of oocytes after cryopreservation and thawing was 35.59%, significant higher than oocytes cryopreservation in GV stages (24.64%) and culture for 24h (23.36%) (P<0.05). The rate of morphological normality after thawing in group of MII stage (72.81%) and culture for 24h (77.22%) was significantly higher than in group of GV stages (53.24%) (P<0.05).