Studies On Pharmacokinetics And Residue Of Chloramphenicol In Perch In Vitro

Chloramphenico1 is one of the antibiotics widely used in aquacuIture. In this study, thepharmacokinetic and residuaI characteristics of Chloraznphnicol in hea1thy Perch are studiedby using the High Performance Liquid Chromatography. In order to provide the theoreticalbases fOr the clinical use of Chloramphnicol, the multiple-dose pharmacokinetic parameterswere assessed according to the single-dose pharmacokinetic parameters. The withdrawal timewas deduced by the elimination of ChloramphnicoI, which must be usefu1 to the residuemonitoring fOr public heaIth.Data were analyzed with the pharrnacokinetic coniputer program MCP-KP. The resultsshowed that the plasma concentration-time course of ChloramphnicoI can be described by aone-compartment open model with the first order absorPtion after oraI administration(80mg/kg). Its theoretical equation was as foIlows: C bItal = 30.8l5 (e -- o.oo14 t - e -- o.bo2 l ), andthe main pharmacokinetic parameters were those: K. 0'662h-l, T l/2K. I '047h, C max 2l '093 llg/mI, T max 3 '96l h, AUC 438.3 l mg.l-'.h-', Kel 0.06l4h-', T,l2k l l .293h.The kinetics process of Chloramphnicol in tissues can all be described by thebiexponential equation with the first order absorption, and the theoreticaI equations and themain pharmacokinetic parameters were as follows fC muscIe = 33'515 (e -- o.os5 t - e - 9'2l9 t ), C max 30'8l5 P g/ml, T m.x 7'079h, AUC662.370mg. l-I .h-',KeI 0.085h-I, T,,,k 8. 1 74h.C li..,= l 8'597 (e -- o.o62 t - e -- l'854t ), C max I 4.23 l u g/ml, T mtx l '894h, AUC 226.9l0mg.I-'.h", Kel0.062h- ', T,,2k I l. l l8h.C kidney = 26'024 (e -- o.o52 l- e -- o.399 l ), C mas 36.037 u g/ml, T m.x 5'889h, AUC 946.07mg.l''.h-', Kel 0.052h", T,,,k l3.424h.The elimination characteristics of muItipIe-dose administrition (40mg/kg) can bedescribed by the equations aS fOllows f C bl- = 3.40 e -- o.4o3 1' C mutcIe = 4'342 e -o.45a l' C liv,l= 2.l52 e -o.239 t' C kideey = 64.l55 e - I'M3 t. According to the equations, the time taken by theconcentrations of Chloramphnicol dropping to 0.0l ll glml in blood. muscle. Iiver and kidneywas l4.l64. l3.26l' 22.476. 6.075 days respectively.The resuItS of phdriacokinetic and residual studies show that after oraI administration,Chloramphnicol can be absorbed quickly. distributed widely and eliminated slowly. The2concentrations in tissues were so high that they are almost higher than the MIC ofChloramphnicoI fOr most bacteriec Acc6rding to the pharmacokinetic parameters, bindingwith the use in the clinic, we suggest the reasonab1e administration project as that the Perchcan be given Chloramphenicol once a day by the oral administration dose of 40mg/kg bodyweight.According to the regular of ChIoraznphenicol elimination, we suggest the withdrawaltime should be about twenty-three days.