Studies On Pharmacokinetics And Residues Of Chloramphenicol And Furazolidone In Apostichopus Japonicus

The pharmacokinetic and residual characteristics of Chloramphenicol (CAP) andFurazolidone (FZD) in Apostichopus japonicus were studied by using the High PerformanceLiquid Chromatography (HPLC). Then we may characterize the metabolic and residual rulesof CAP and FZD in A. japonicus to control drug abuse in aquaculture, and to minimize thepotential risks for human health and environment. The main results are as followed:1. Coelomic fluid, respiratory trees, body wall and muscle were sampled after coelomicinjecting with a single dose of CAP (20mg/kg). The results showed that theconcentration-time course of CAP in body wall could be described by a one-compartmentmodel, and the course in coelomic fluid, muscle and respiratory trees could be described by atwo-compartment model. The main pharmacokinetic parameters were as follows: theelimination half-time (T1/2β) were13.58,13.59,16.11,10.90h in coelomic fluid, respiratorytrees, body wall and muscle, respectively; the peak concentration (Cmax) were28.19,37.22,6.84and33.89μg/ml; the peak time (Tmax) were0.15,1.10,1.49and1.31h; the area under theconcentration-time curve (AUC) were162.76,328.24,169.48and350.28μg·h/ml. Theelimination characteristics of multiple-dose administration (10mg/kg) could be described bythe equations as follows: Ccoelomic fluid=2.0586e-1.2747t, Crespiratory trees=1.4727e-0.9164t, Cbodywall=0.9184e-0.1058t, Cmuscle=0.697e-0.6619t, and T1/2βwere0.54,0.76,6.55and1.05days. Thesefindings indicated that Chloramphenicol could be fast absorbed in A. japonicus after injection,while it was slowly metabolized with a long elimination half-time, especially, remainedheavily in body wall.2. Coelomic fluid, respiratory trees, body wall and muscle were sampled after coelomicinjecting with a single dose of FZD (10mg/kg). The results showed that theconcentration-time course of FZD in body wall could be described by a one-compartmentmodel, and the course in coelomic fluid, muscle and respiratory trees could be described by atwo-compartment model. The main pharmacokinetic parameters were as follows: theelimination half-time (T1/2β) were27.86,24.54,4.71,66.70h in coelomic fluid, respiratorytrees, body wall and muscle, respectively; the peak concentration (Cmax) were3.43,1.39,0.62and1.34μg/ml; the peak time (Tmax) were0.18,0.08,0.78and1.13h; the area under theconcentration-time curve (AUC) were17.30,14.36,4.75and16.97μg·h/ml. The eliminationcharacteristics of multiple-dose administration (5mg/kg) could be described by the equationsas follows: Ccoelomic fluid=0.0848e-0.0213t, Crespiratory trees=0.1298e-0.0621t, Cbody wall=0.1167e-0.1003t,Cmuscle=0.4078e-0.0699t; and T1/2βwere32.54,11.16,6.91and9.91days. These findingsindicated that Furazolidone could be fast absorbed in A. japonicus after injection, while it was slowly metabolized with a long time. The residual concentration in A. japonicus could still bedetected after25days.