| The study on the biochemical analysis and the effect on the immune function of mice of low molecular weight extract(MW<10ku) derived from bovine colostrum.In this experiment we have conducted the research on the biochemistry character and the effect of on the immune function of mice of low molecular weight extract (MW<10ku) derived from bovine colostrum. The results of the experiments can serve as the basic data and theoretic foundation to large colostrums food marketing.In experiment I , the made-method and biochemistry assay of low molecular weight extract from bovine colostrum follows as : colostrum of five Chinese Holstein cows was Centrifugal lipid removal with 4000r/min for 40min. then we gain colostrum whey by 0.1% rennet was added to skim milk, colostrum whey permeates of UF treatment with lOOku mw cut off, top it off, whey permeates of UF treatment with lOku mw cut off again.By means of test, the properties of low molecular weight extract (LMWE) follows as: the concentration of Peptides is 2.1mg/ml; the percentage of lactose is 4.3% and the percentage of CN is 0.022%. When 20% sulfosalicy acid was added into low molecular weight extract, there is no insoluble precipitate . low molecular weight extract contains 18 kinds of amino acids, particularly ,the concentration of Glutamic acid is the highest1.52mg/ml. After diluted 6 times, the OD value of the low molecular weight extract by 755 spectrophotometer follow as :OD25o is 1.056; OD26o is 1.276; OD2g0 is 0.6; OD26o/OD280is2.12.In experiment II, immune activities of low molecular weight extract on mice were observed.Forty mice were chosen and randomly divided into controK A, B-, C four groups on average. Mice in A^ EK C groups were respectively given lOg/kg, 25g/kg, 50g/kg of low molecular weight extract and control by subcutaneous injection saline for seven days. The result exhibited dose depending relation, higher dose (0.5ml/mice and 1 ml/mice) low molecular weight extract could significantly improve the phagocytosising function of macrophage in abdominal cavity (p<0.01), lower dose(0.2ml/mice) had no effect on mice. The concentration of blood MDA of treated groups were lower than that of control (p<0.01) and didn't exhibit dose-dependent pattern. The administration of low molecular weight extract could strongly affect ANAE(+) cell percentage of peripheral blood lymphocytes and improve the ratio of lymphocyte and neutrophile(p<0.01).Cyclophosphamide model mice in group A^ B-. C were subcutaneously injected at doses 10g/kg> 25g/kg> 50g/kg of low molecular weight extract respectively and control by subcutaneous injection saline for lOdays. the results showed that lower dose(10g/kg) low molecular weight extract could notably strengthen the delayed type hypersensitivity(DTH) caused by DNCB text model in mice(p<0.01), Higher dose(25g/kg and 50g/kg) had no effect, low molecular weight extract could significantly restore the formation of hemolysin antibody of sheep red blood cell text model which was inhibited by cyclophosphamide(p<0.01) and had no dose-depend pattern.Forty mice were chosen and randomly divided into 4 groups on average, which were respectively subcutaneously injected 10g/kg> 25g/kg. 50g/kg low molecular weight extract and control were respectively subcutaneously injected saline by subcutaneous injection for ten days. The result indicate that low molecular weight extract could remarkably enhanced the content of DNA and RNA of thymus in treated mice(p<0.01) compared with control group. It was suggested that the immunomodulatory mechanism of low molecular weigh extract was associated with its promotion of the synthesis of DNA and RNA. Low molecular weight extract had no effect on the weight of thymus , spleen, liver and adrenal-gland. The activity of blood GSH-Px and SOD could significantly increase in mice in treated groups(p<0.01) and exhibited dose-dependent pattern. Low molecular weight extract could delay swim time, but the result wasn't obvious.The conclusion can be deduced from our results that: low molecular we...
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