| In order to investigate the mechanism how to regulate the host's immunity by Nasonia nitripennis ,this article makes the preliminary study in the following topics: the structure of venom gland, change in constitution and count of hemocyte population, and the concentration of soluble proteins and soluble sugars after parasitization. The result is summarized as following:There are two kinds of hemocyte in hemolymph of Boettcherisca peregrina pupae, plasmatoctes(PLs) and granular cells(GRs). In the first day after parasitization, the percentage of PLs is significantly higher in parasitized pupae than unparasitized ones, so as the percentage of GRs. But from the second day to the fifth day after parasitized, the percentage of GRs and PLs aren't significantly different between the parasitized pupaes and in unparasitized pupaes.From the first day to the fifth day after parasitized, the count of GRs and PLs is significantly higher in parasitized pupaes than in unparasitized pupaes. After the first day parasitzed, total hemocyte count (THC) isn't significantly different between parasitized pupaes and unparasitized ones, and from the second day to the fifth day, it is significantly higher in parasitized pupaes than unparasitized ones, and THC of parasitized pupaes is 3.2, 1.8, 2.1 and 2.3 times than that of unparasitized pupaes.The venom reservoir is consisted of an outer musular sheath, a single layer of secretory cells and an inner single layer of squamous cells adjacents to the intima which lines the lumen. The intima invaginates into each secretory cell and forms a duct which is surrounded by duct cells. And the gland filaments consist of a single layer of cuboidal secretory cells and an inner layer of squamous cells adjacent to the intima which lines the lumen. The reservoir apparatus are bordered distally by longitudinal muscles which form a relatively thick muscular sheath, and are innervated, the rapid injection of the venom may be facilitated by muscular contraction of the reservoir. In the gland filaments, the secretory cells of venom apparatus are cuboidal and form a continuous layer which is limited basically by a granular lamella. Nuclei of secretory cells are relatively large and contain scatteredchromatin. Concentration of sER and Golgi bodies around the secretory appartus indicate the possibility of active transport through the secretory appartus. But there can't be seen in the reservoir's cells. From the fact above, we can make a conclusion that the secretory function mainly acted by the gland filament, and the main function of reservoir is storing the venom.From the utilization of the software PDQuest檛o exmaine the photo of venom's electrophoresis, there are 100 more protein dots can be deteced. The protein's weight are from 2 KDa to 145 KDa, the dots mainly scattered in the weight from 2 KDa to 40 KDa, only several dots above the 70 KDa. Using 2-DE we know that venom protein are mainly acid protein, and molecular weight is mainly between 2-40 KDa.From the zero point fifth day to the sixth day, the concentration of venom is ascending, and there comes the highest concentration. But on the seventh day, the concentration is sharply droping, and high lower than the concentration of sixth day.The concentration of protein is relatively stably in the hemolymph from the first day to the fourth day, changing between 22.95mg/ml and 43. 51mg/ml, it is is 3.2, 1.8, 2.1, 2.3 times higher in unparasitized pupaes than the in parasitized pupaes. The concentration of protein in the hemolymph from the unparasitized pupae is droping from the first day to the fourth day. The concentration of protein in the hemolymph from parasitized pupaes are droping from the first day to the third day, but on the fourth day , concentration are rising. The concentration of the protein of 100 KDa is lower in the hemolymph of former stage, the concentration is rising on the third day in the parasitized pupaes, but rising on the fourth day in the unparasitized pupaes, much lower than that in parasitized pupaes. The c... |
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