Transgenic Organism Selectable Maker Gene And Control Gene Clone And Detection Technology Research

With commercialism of transgenic organism-Genetically Modified Organism(GMO) increasing, transgenic technology of organism will bring a thorough novelty for agriculture. At the same time, the safety problem of transgenic organism became more and more important and many people have their own different points.Because of these reasons, the detection of transgenic products has became indispensable and necessary. Their detection has become a key to gaining the enough research space and better market prospect.Selected Dongnong 1028 maize breed cultivated in North East Agricultural , abstracted the total DNA, and psmRS-GFPCD327 plasmid as template, primers were designed according to the punished sequence of Genebank-action gene of zea mays^ streptomycin and hygromycin marker genes with Primer 5.0 and Generunner software ,connected DNA with pMD 18-T or pUCIS vector, transformed them into JM109 bacterium, obtained the clones. Abstracted the recombinant, and identified by corresponding restriction endonuclease and PCR.. It is indicated that the three genes have been cloned successfully.Selected many kinds of plants, abstracted the total DNA by CTAB method, the three genes were Labeled with DIG nonradiactive, detected transgenic products by PCR and dot blotting.