Transformation Of Tobacco With The Gene Of CspB

In our country, drought is one of the most important stress factors for productivity of crops. Breeding of drought-resistance crops are regarded as the most efficient strategy to utilize drought soil. However, traditional breeding technologies to improve drought resistance of plants are time consuming and low efficient methods. With the development of molecular biotechnology and understanding of drought-tolerance mechanism, more and more drought-resistance genes have been found. These genes have been applied to improve drought resistance of crops via transgenic plants.CSPs play an important role both in plants and bacteria under stress environment. CSPs function as transcriptional anti-terminators or translational enhancers at low temperature, drought and salt stress by destabilizing RNA secondary structure, to ensure the translation progress as normal. Simultaneously,CSPs also can improve the capacity of light-harvesting when plants are in drought-resistance.CspB is a member of the family of CSPs. In order to study cspB gene function in stress tolerance of plants, 5 transgenic tobacco plants were developed with cspB by the Agrobacterium-mediated transformation, transgenic plants were identified by antibiotic selection,RT-PCR and Southern blotting, Photosynthetic rate of the transgenic tobaccos and wild tobaccos were tested in different conditions. The result is that photosynthetic rate of transgenic tobacco is better than of wild type in drought stress.