Studies On Biological Characters Of Lilium And Cross-Incompatibility Between Distantly Related Species

Lilium is one of the famous flowers of the world. It is used as a cut flower, a potted flowering crop and a garden ornament. So people specially love it. It has very high values in commerce and ornament as well as the edible and medical values. In the flower market of our country, regardless of the fragrant Oriental hybrids and L.longiflorum Thumb as well as the simple and unadorned Asiatic hybrids. Which are cultivated by foreigners. Moreover, the cold extremely zones in our country have the worse cut flower species which are fit to be planted outdoor. In the resistant cold breeding of the lilium breeding, there are not new breakthrough for many years. A lot of problems have not been resolved. In HEILONGJIANG province. There is a wild species-Lilium lancifolium, which has many special characters (especially frigostable property) which other cultural lilium cannot have. So people pay more attention to the L. lancifolium.This experiment is based on comprehending the biological character of lilium, Explore hybridisation between distantly related species of Lilium such as lilium lancifoliunu Asiatic hybrid , Oriential hybrid .including cross-compatibility mechanismThe experiment's main aims are to explore distant hybrid of the lilium and genetic mechanism to gain species of the resistant cold to offer the basic theory foundation for resolving the breeding difficulties of the lilium. The results are as follows:l.The morphological feature ofLiliumIt is not distinctly different between the seed of the distant crossing of lilium and the seed of the crossing parents of the lilium.Observed under the electron microscope, the stigma of the lancifolium hadn't dehisced. However, the stigmas of the Asiatic hybrids and oriental hybrids had dehisced.2. In the pollen segregation germination culture medium are sucrose 13%, boric acid 143mg/kg, agar 1%.3. The six species hybridisations (direct cross and reciprocal cross) of the Lancifolium and the Oriental hybrids are compatible. The imcompatibility of direct cross and reciprocal cross cause, during the course of pollenody and pollen tube extenence, the pollens' germination rate of the stigma is low. When the pollen tube which had extended into the style, grow by 1/3 of 1/2 of the style. It will have to stop growing during to the callose deposit. Sothe anisomerogamy hadn't caused.4. However, the hybridisations of the Asiatic hybrids and Oriental hybrids are compatible, but both compatible courses delay slowlier 3-4 days than both separately auto gamic courses, and all the courses delay slowlier 10 days than the autogamy.S.When larva embryo segregation culture after hybridisations the addition of MS culture medium are 0.01mg/1 NAA. This culture medium is the best.While culturing larva embryos which are induced and survive for 40days, but the induction rate isn't high. The longer the stadiums are, the higher the induction rate is. While culturing embryos, the stadium isn't distinctly different for euphylis and root differentiations. But it is influent for scales formation, the stadium increasing, the number of the scales will raise.Graduate: Fan jinpingMajor: pomologySupervisor: associate prof.Che daidi...