| The experiment was conducted to study the effect of different Oligosaccharides on performance, intestinal bacteria population, availability of nutrition and metabolism of cholesterol by feeding trial, metabolic trial and slaughter trial.Feeding trial: 144 Avin broilers (1d of age) were randomly divided into six groups, with three replications per group, whose weights were similar among treatment groups. The control group (Group I) was fed with corn-soybean meal basal diet, the trial groups (Group II, III, IV,V and VI) with basal diet plus 0.1% Manoligosaccharides (MOS), 0.3% Soybean-Oligosaccharides (SBOS), 0.3% Fructooligosaccharides (FOS), 0.3% a -Glucooligosaccharides ( -GOS) and 0.03% Chlortetracycline (CTC), respectively. The raising period is seven weeks. Weights, gains and feed intakes of every replication were weight every weekend, and average daily gains, feed intakes and feed efficiency were calculated. The result showed that Oligosaccharides supplement improved daily gains and feed intakes (P> 0.05) during the whole growing stage, and there is no difference between trial groups (P> 0.05). Feed intakes in SBOS were higher 5.55% (P>0.05) than in the control group. CTC supplement significantly (P<0.05)decreased growths and feed intakes and were lower 9.75% and 9.51% than the control group, respectively. During every raising stage, Oligosaccharides supplement improved growths and feed intakes of young broilers, whose weights of 21d of age were higher than the control group, but had no effect on growths of adult ones. Oligosaccharides supplement promoted growth of young broilers and had no effect on adult ones, which compensated for promotion earlier stage so that it didn't express promotion throughout the growing stage.Metabolic trial: Metabolic trial began from 17d to 21d. The result showed that SBOS and FOS supplement improved availability of energy (P>0.05), but MOS and a -GOS supplement significantly (P<0.05) decreased. Oligosaccharides supplement had no effect on availability of protein and improved the availability of phosphorus, calcium, magnesium and iron. The availability of phosphorus of FOS group was higher than that of a -GOS and the control group. SBOS supplement increased significantly the availability of iron.Slaughter trial: At 21d, one broiler was slaughtered each group, and fresh cecal contentswere collected and analyzed for Bifidobacterium and Escherichia coli concentration. Cecal were weight and measured their length. Thorax, spleen and Bursa of Fabricius of broilers were weight respectively to measured indexes of immune organs. At 21 d, 35d and 49d, blood samples were obtained from one broiler of each treatment group to measured serum cholesterol concentration, and at 49d, one broiler in each group was slaughtered and gained muscle to measured cholesterol concentration. The result showed that oligosaccharides supplement increased significantly (P<0.05) Bifidobacterium concentration. MOS, SBOS and FOS supplement decreased Escherichia coli concentration, but -GOS increased Escherichia coli concentration. Bifidobacterium concentration of CTCgroup was significantly lower than that of other groups (P<0.05). At 42d, oligosaccharides supplement had no effect on Bifidobacterium and Escherichia coli concentration. There was no difference between oligosaccharides groups (P>0.05). Oligosaccharides supplement only increased Bifidobacterium concentration of young broilers and had no effect on adult ones. FOS and -GOS supplement significantly increased cecal weights of broilers of 21 d of age and higher than MOS and SBOS. Oligosaccharides supplement had no effect on weights of cecal of adult broilers and had similary effect on the length of cecal. Oligosaccharides supplement didn't affect indexes of immune organs, cholesterol in serum and chicken, and there were no difference between them. But Oligosaccharides supplement increased cholesterol in fecal, and SBOS group was the highest, higher 14.91%(P<0.05) than a -GOS group, and there were no difference among other oligosaccharid...
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