Study On Plantlet Regeneration Of Paulownia Plants Via Their Somatic Embryogenesis

Paulownia is a kind of popular, fast-growing trees which are of good quality,but these problems.such as witches broom, large crown and shout main stem obstruct Paulownia further denelopmento With the boom of modern science and technology, gene engineering and cell engineering provide effective methods to solve these problems.Research about the plantlet regeneration is basic for application of plants modern technology, between the two ways by which plantlets regenerate, somatic embryogenesis is more superior, it not only set up ideal systems for research about cell differeciation , morphogenesis etc, but also is necessary to study and make man-made seeds.Somatic embryogenesis and plantlet regeneration of Paulownia lay sound foundation for research on gene engineering, cell engineering and cultivation of new kind Paulownia, etc.having studied the somatic embryogenesis and plantlets regeneration from leaves and stem segments of Paulownia.tomentosa, Paulownia.elongata and Paulownia.fortunei, we come to such conclusions. 1. The optimum media for cell embyogenetic callus from differentexplants of three kinds of Paulownia respectively were: leaves of Paulownia.elongata MS+0.3NAA+8BA, stem segments of P.elongata MS+0.3NAA+11BA, leaves of P. fortunei MS+0.3NAA+8BA, stem segments of P.fortunei MS+0.3NAA+14BA, leaves of P.tomentosa MS+0.3NAA+17BA, stem segments of P.tomentosa MS+0.3NAA+17B A.2. Stem segments and leaves of P.elongata which is the progeny of P.fortunei and P.tomentosa were respectively similar to leaves of P.tomentosa and stem segments of P.fortunei in conditions under which embryogenetic callus were induced ,but to certain degree were different in tha law of somatic embryogenesis of different kinds of Paulownia.3. Somatic embryogenesis of different Paulownia experienced the same courses: early proembryo, globular embryo, heart-shaped embryo,torpe-do-shaped embryo and cotyledonary embryo. Under the experimental conditions ,the somatic embryogenesis of Paulownia mainly originated in indirect way. On the same media were found the induction of embryogenetic callus, embryogenesis of all phrases, even maturatiom and normal transformation of somatic embryo.