Studies On The Diarrhoea Etiology Of The Weaned Young Rabbits And Its Prophylaxis

This research was carried out according to the diarrhoea question in the weaned young rabbits .According to the microecology theory, we studied the microbiota in the intestine of the weaned rabbits with the microbiological methods, and we selected and identified the aerobes (Escherichia.coli, Sta.aureus and Yeast,et al)and anaerobes (Lactobacillus, Bifidobacterium, Bacteroides, Fusobacterium, and Peptococcus,et al) in the healthy and the diarrheic weaned rabbits correspondly.Through the study, we intended to find the regulation and cause to the diarrhoea of the young rabbits, and give some solution to the control to the young rabbits' diarrhoea.In the identification of the anaerobes, we identified 26 strains anaerobes by the gas chromatography(GC), which is a accurately and quickly identification method to anaerobes. Also, by using the microbe preparation fermented feed to rear the young weaned rabbits, we found the results are good and satisfied.This study consists of three parts:PartⅠ:the study of the change of microbiota in the healthy and the diarrheic weaned rabbits'intestinesUsing the general purpose media and the media for the selective isolation of bacteria, we can isolated and selected the bacteria in the intestines. The results showed, in the intestines of healthy rabbits, the concentration of the total number of aerobes is 4.37×104 CFU/g, that of Yeast is 1.78×104 CFU/g, that of Enterobacteriaceae is 1.23×104 CFU/g, and that of Str.aureus is 6.92×103 CFU/g；The concentration of total anaerobes is 5.25×106 CFU/g,that of Bifidobacterium is 2.09×106, Bacteroides is 1.86×106 and Lactobacillus is 8.71×105, et al. In the diarrheic rabbits,the total numbers of aerobes is 3.16×106 CFU/g ,that of Enterobacteriaceae is 1.38×106 CFU/g,et al. The concentration of total anaerobes is 3.16×106 CFU/g ,that of Clostridium is 9.12×105,that of Bifidobacterium is 5.75×105CFU/g, et al.Compared with the concentration of the healthy rabbits, the concentration of anaerobes and Enterobacteriaceae exhibited statistical highly statistical significance ( P<0.01). The concentration of Yeast and Str.aureus between the healthy and diarrheic rabbits had no statistical significance (P>0.05). The aerobes in Duodenum, Ileum and Rectum had statistical significance (P>0.05).Analysising the anaerobes concentration in every intestine between the healthy and the diarrheic rabbits, only that in the Caecum had highly statistical significance (P<0.01),the Ileum had statistical significance (P>0.05), the others had no statistical significance(P>0.05).The concerntation of Bacteroides showed highly statistical significance(P<0.01), the other anaerobes showed no statistical significance to the healthy rabbits (P>0.05).The ratio of aerobes and anaerobes between diffrent intestines had highly statistical significance (P<0.01).Part Ⅱ:Identification of the anaerobes with GCThe 26 strains anaerobes selected from the rabbits in the study of part Ⅰ, was cultured in PYG media, and processed to get the volatility fatty acids and the non- volatility fatty acids which can identify the anaerobes. There were Lactobacillus 7 strains, Bacteroides.fragilis 6strains, Fusobacterium.nucieatum 4 strains, Eubacterium.moniliforme 4 strains, Bifidobacterium 2 strains, Clostridium.novyi 2 strains and Peptostreptococcus. tetradius 1strain . The results showed that GC is a quickly and accurately method to the identication of anaerobes, which can get variable use in the research and diagnose of the infectious diseases.Part Ⅲ:The application of microbe preparation in weaned young rabbitsFour hundred and fifty Hyla rabbits of weaned were submitted to one of three treatments. Treatment one was eated with the forage added with the microbe preparationⅠ,and treatment two was eated with the forage added with the microbe preparationⅡ,and the forage in treatment three was added with the pharmaceuticals. The results showed that the microbe preparationⅠhad the great promote effect to the young rabbits'survival rate, th...