| There wre many kind of strawberry fungal diseases in China.Among all of fungal disease, strawberry Botrytis cinerea Pers, which was provoke by Botrytis cinerea Pers.ex Fr, was comparatively the most wide-spread and relatively the most harmful ones. Botrytis cinerea Pers.ex Fr can invade other horticultural plant else. What's more,it has been single resistant, double resistant, even multi resistant. So controlling this kind of diseases is hardy undertaking. It is important that strawberry is direct eat and pesticide residue is very sensitivity. Through screening and utilizing profitable fastidious controlling Botrytis cinerea Pers is increasingly important means. Trichoderma has superior biocontrol character. Through ion beam influxing Trichoderma and sceening highly antagonizing Botrytis cinerea Pers is new and available way.Trichoderma belongs to Deuteromycotina - Hyphomycetes , Hyphomycetales -Hyphomycetaceae, Trichodermapers.ex Fr.It extensively exists in ecological environment of soil , rhizoplanex surface of leaf, seeds and bulbodium etc.In 1932,Wanding first discovered that Trichoderma lingnorm can parasitize many kind of pathogenic fungus in many plants. So he proposed that Trichoderma should be used in biologically controlling soil-borne disease of plant. The development work about biological control effects of Trichoderma was stared from this time. What's more, Trichoderma has abroad adaptability, broad spectrum and multi- mechanism, so it is emphatically researched by many scholars who studied biological control of plant disease.Along with development of biotechnology, Trichoderma has been started to purposefully utilize biotechnology to transform and gain new type of strain, but it was only separated and selected from nature in the past. Now for example making use of ultraviolet radiation irradiation to get induction mutation, protoplast fusion and genie transformation technology, we could research and develop new-style Trichoderma strain.Optimizing mutagenic strain of Trichoderma that can high antagonize strawberry Botrytis cinerea Pers is T90-1 through ion beam implanting into Trichoderma to make genetic material generate mutation. Then the size of colony that mutagenic strain cultured on clitin cultivation medium, the number of producing spore, the measure of mill offals fermenting liquor and the value of inhibition to Botrytis cinerea Pers etc were four waysscreening.T90-1 and Botrytis cinerea Pers confront each other on cultivation medium . When T90-1 made contact with Botrytis cinerea Pers and subsequently the pathogen colonies were either overgrown or invaded by T90-1, leading pathogen to death. Zymolytic liquid of T90-1 can decompose mycelial wall that account for T90-1 excreteing chitinolytic enzymes during zymolysis.50X diluent of fermenting liquor can decompose mycelial wall of Botrytis cinerea Pers within 8 hours and let protoplast denudate.The spore and fermenting liquor of T90-1 can not only restrain Botrytis cinerea Pers but also increase disease resistance of strawberry. After spraying the spore and fermenting liquor of T90-1 on leaves of strawberry, enzyme activity of peroxidase and polyphenoloxidase were improved through analyzing isozyme of gel electrophoresis and ultraviolet spectrophotometer checking and measuring enzyme activity.T90-1 was continuously resetted on PDA cultivation medium eight weeks. Every week detecting the size of colony that mutagenic strain cultured on clitin cultivation medium , the number of producing spore, the measure of mill offals fermenting liquor, statistical results indicated that degree of separation is very little and odds is no markedness. Fermenting liquor was keeped in normal temperature(25) eight weeks and detected enzyme activity every week. Statistical results indicated that the standard deviation of enzyme activity is only 0.393%. So diversity is little and almost no variation.In addition, fermenting liquor of T90-1 had preferable effect on keeping strawberry fresh and could inhibite Botrytis ciner...
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