Effects Of Active Immunization Against Cholecystokinin On Endocrine And Pancreatic Function In Pigs

This study explored the endocrine and pancreas responses to active immunization against cholecystokinin-octapeptide (CCK-8) in pigs. Five young growing pigs were immunized with CCK-8 conjugated to human serum albumin (HSA). Primary inoculation was on d 1. Booster inoculations were on d 29, 43 and 64. An equal number of control animals were immunized against HSA by the same protocol. All pigs were slaughtered on d 77. Blood samples were collected on d 1, 15, 29, 43, 57 and 71. Data indicated that the antibodies were raised in CCK- treated group. Feed intake was 16.23%(p=0.152) greater and body weight gain was 21.23% (p=0.104)greater for the CCK than for the HSA-treated group. The development of gallbladder and pancreas were not affected by CCK-8 immunization(p = 0. 932, p=0. 144). The specific activity of trypsin was increased(p=0.22) in CCK-treated group. Active immunization decreased the concentration of insulin and CCK in serum(p<0.05), but had no effect to growth hormone in serum(p>0. 05) ,the concentration of leptin tended to decrease. In conclusion, active immunization against CCK-8 resulted in development of high antibodies, accelerated growth and affected the secretion of some hormones, but failed to cause negative influence to the function of gallbladder and pancreas.