The Viruses Elimination And Microtuber Induction Of Potato

Aiming at establishing a method of meristem tip culture, virus detection and virus free seedling multiplication ,two cultivars of potato, KexinS and Deyou 1,which were highly susceptible to potato PLRV and PYMV ,were chosen as explants in experiments. The results were as follows:1) The explants with two phyllomes(about 0.2-0.5mm in size )were found to be suitable for virus-free seedling culture , which were cultured on solid MS medium supplement with 6-BA,NAA and IAA ,The result indicated that the plantlet was over 30% after three months. In addition, a 4-week heat treatment(37℃)to seedlings before cutting down the meristem tip could result in a higher virus-free ratio and survival ratio almost has no change .2) It will not to be polluted easily in the subculture on solid MS medium , but the seedlings were weaker .A fiber carrier liquid medium was not only suitable for strengthening seedlings but also could result in a higher proliferation ratio .3) Using 150mL triangular flask with Parafilm with the combination of liquid culture, cycocel and light intensity changing were effective in eliminating flask seedling weakness which can affect microtuber production.4) Adding 3-5mg/L 6-BA or 500mg/L CCC to the medium, keeping it 8 hours in light and 16 hours in darkness every day could obviously improve microtuber production5) Up to over 95% of virus-free seedlings could survive when the seedlings with a length of 8cm were put into the air at 20℃ for 2 days.