| In this experiment, the lump and cultural plantlets of potato varieties includingGuangyin No.1, Guangyin NO.2, Guangyin No.3, Guangyin No.4 and Sishu No.1 wereused as the experimental materials. The system of tissue culture and microtuberinduction was studied and the tissue culture and rapid propagation system of 5 newpotato varieties was established. Some cultural plantlets and microtuber had beenobtained successfully. The results offered the materials, test basic and science referencefor the microtuber formation in the further research work. The experimental resultsindicated that:1,After sterilized the tuber and bud explants of potato, the best culture mediumwas MS.2,The combination of 5％NaCLO 3min+0.1％HgCL2 7min+70％CH3CH2OH0.25min was suitable to sterile the buds of potato, the contamination rate was 3.16％, thehighest average number of bud was 2.54, and the bud growing was the best.3,6-BA,NAA could promote the buds induction and differentiation. The optimalinduction medium was MS+6-BA0.5mg·L-1+NAA0.3mg·L-1+Sucros30g·L-1+0.1％A.C.The rate of buds differentiation was 73.53％, the plantlets was thick and tall, came outevenly. The height of plant was 5.81cm, and the highest rate of root was 73.53％.4,Carried on systematic research on the influence of plantlets multiplicationabout concentration of sugar, hormones(6-BA, NAA, KT, Met, Bg).The results showedthat sugar concentration with 30g·L-1had optimal effect on the plantlet subculture, 6-BA and Met gave advantage function on the plantlet multiplication, KT was notsuitable to the plantlet multiplication. The suitable concentration of B9 could shorten therange of stem, increased the thick of stem and raised the plantlet quality.5,In the medium of plantlets subculture, adding 25mg·L-1 CM in the media wouldbenefit to the plantlet multiplication. The optimal subculture multiplication medium wasMS+6-BA1.0mg·L-1+NAA0.5mg·L-1+Met1.0mg·L-1+B915mg·L-1+CM25ml·L-1.Theaverage number of plantlets multiplication achieved to 3.07.6,The optimal basic medium of microtuber induction was MS.7,Different hormone combination gave different responses to the effects ofmicrotuber induction. The optimal hormone combination for microtuber induction was6-BA0.5mg·L-1+NAA0.3mg·L-1+Coumarin25mg·L-1.Among 5 potato varieties, SishuNo.1 had no microtuber.8,Sugar with 8％concentration was advantage to the microtuber induction, andthe microtuber was bigger. AC with 0.1％concentration could promote the tuberization.9,Different light condition gave different effects on microtuber induction. Theculture of dark alternates with light was best ways to microtuber induction.10,Different position of materials gave different effects on microtuber induction.The basilar section of stem was stronger than the top section and easier to inducemicrotuber, and the quality of microtuber was the best.11,The effect of root induction was notable when added the suitable concentrationof ABT to the medium. In the culture of root induction and sound seeding, the optimalmedium was: MS+6-BA0.5mg·L-1+NAA1.0mg.L-1+Met2.0mg·L-1+B920mg·L-1+ABT2.0mg·L-1. The average of root induction rate was 94.10％,the number of root was 5.16.12,In the medium of root induction and sound seeding of potato plantlet, adding30mg·L-1 Rifampicin in the media could promote the root thick.13,Different matrixes showed different effects on plantlet transplanting andmicrotuber formation. The optimal effect of transplanting was(humus soil: peatysoil: river sand=1: 1: 1).14,In different ways of transplant on the plantlets, the way of transplant with singleplant and root could promote the survival rate, and the plants grew well.
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