| The deer and mankind's life have had close relations since ancient times. The deer is the treasure all over, has provided the extremely abundant products for people, among them the most valuable products are the pilose antler, It is the traditional Chinese medicine with famous and precious tradition, can treat many kinds of diseases , more than one hundred kinds of Chinese patent drugs all have pilose antler compatibilities.Our country raise deer most early country in the world , raise and train Strain of deer oldest country too, Our country raise and train sika have a history of more than 300 years already, our country editions of the People's Republic of China pharmacopoeia" stipulate sika only, red deer the crude drags of normal product in 2000 among them. Fall sharply because of pilose antler and products resource demand increase sharply, make false product, obscure product and constant to emerge substituteHave the problem that the serious one pass a fake product off as a genuine one and adulterate according to domestic market, The security of restricting traditional Chinese medicine to a great extent is effective and to the modernization, standardized and internationalized development, Variety its determine thorny their determine that has an important realistic meaning more and more with quality appraisal.Traditional crude drug determine mainly including base source determine, properties determine, determine while being micro, the physics and chemistry are appraised, the living beings determine etc.. Still important content and means that pharmacognosy study so farAdopt the molecular biology qualification method in this research, increase the SRY genes of four kinds of Strain of deer, Guide thing distinguishing from what vision that increase can see we adopt to these 4 kinds of pilose antler accurate. Guide with this kind thing increase four deer these may increase and produce the obvious strip under the same condition And there are differences in the figure of strip.And negative contrast appear any strip prove the result ture and reliable! We plan and spend and guide 2 thing in this way, TAQ enzyme, dNTP, PCR reacts and buffers the liquid Make up four crude drags reagent box that pilose antler determine these Make for standard with increased above vision, can effective to examine and produce kind of pilose antler, Because difference and report that difference go on of colony in the kind in deer yet at present, This reagent development of box, can prevent the homology from imitating the overflowing of crude drags effectively , Whether it maintain economic benefits of people,with technology it is again in practice it is the constant to use and not getting more perfect day by day, Can set up the detection technique of crude drags of famous and precious similar pilose antler . Among molecule research that system need of vertebrate, accumulate in a large amount with mtDNA array. People expecting nuclear information of DNA more and more urgent, come materials in mtDNA supplement, Whether in order to learning at system among not studying to speaking and targetevery one for knowing all-sidedly and accurately. Because base of rDNA order can as study biosystem evolve and reliable object of reference that classify, Research this come and probe into through 18SrDNA part heredity of animal, department of deer, split up the relation, Molecule system learn relation of evolving.Clone and check order to China four Strain of deer ribose 18 SrDNAs of body of deer among experiment, On sending to GeneBank( sequence number is separately: AY225108 AY223903 AY 145527) compare homology among its species to study, Adopt DNASTAR MegAlign software of software package arrange in an order according to Clustal, spend Mega 2.1 UPGMA law achievement of software. Adopt UPGMA law, Bootstrap examine, result and Mega 2.1 NJ law of software its structure and evolve tree unanimous. Draw the red deer and split up from the sika finally . Their homology is the highest. Keep the same with results of study, such as Wang& Du(...
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