Beet M14 Strain Of Molecular Markers

Sugar beet M14 (VV+1C, 2n =18+1) is a monosomic additional line of Beta corolliflora (CCCC, 2n = 4x = 36) in B.vulgaris (VV, 2n = 18), which was obtained from the intercross of the two species(Beta corolliflora ×B.vulgaris). In order to distinguish the hybrid M14 from local species B.vulgaris, DNA was extracted from sixteen hybrids M14 and two local species B.vulgaris and analyzed by Random amplified polymorphic DNA (RAPD) and Amplified fragment length polymorphism (AFLP) techniques.In this work, forty-three primers were used for DNA amplification in RAPD. Sixteen primers were used in AFLP analysis. The results showed that three or five distinct fragments were amplified from the hybrid M14 DNA samples in RAPD or AFLP analysis, respectively. These distinct fragments could be used as potential molecular markers to distinguish the hybrid M14 from local species B.vulgaris. At the same time, the analysis of cluster and genetic similarity coefficient was conducted among all the materials used.