An Ipt Gene Driven Under The Rice Endosperm-Specific Promoters Expressed In Transgenic Tobacco Plants

The rice endosperm-specific expressing promoter, prolamin promoter and glutelin promoter were cloned from rice total DNA by PCR, these two promoters fused to the beta-glucuronidase(GUS) repoter gene in binary expression vector pBI121 respectively, named pBBP and pBIG; then, we used ipt gene which was isolated from A.tumefaciens represented the gus gene of the vectors pBIP, pBIG and pBI121, named pBIPI, pBIGI and pBI35I, As this, we obtained five recombined vectors . These vectors were introduced into tobacco plants by the Agrobactenum mediated leaf-disk method. After transformation, some plants which can grow up in the Km selective medium identified by PCR have the aim band, and sequence analysis conformed that the fused genes have integrated into the genomes of tobacco plants. Histochemical analysis revealed that the Gus activity was restricted to the endosperm tissue. Cytokinin level in seeds was determined by ELISA kit, the result showed that the iPAs level in transgenic seeds were increased by about 4.17and 2.43 fold of the CK (control) , the ipt gene driven under the rice endosperm-specific promoters have specific expressed in the transgenic tobacco plants. Furthermore, statistical comparision between the transgenic plants' and CK' seeds weight indicated that the transgenic seeds are heavier 12.14% and 7.8% than CK'.