The Clone Of Syngrapha Falcifera Multiple Nuclear Polyhedrosis Virus-D DNA Fragments

The experiment has two parts.one is the endonucleases analysis of SfaNPV-D clone;the other is the molecular clone of SfaNPV-D clone EcoR I fragments.In the second part,to use plasmid pUC18 as vector.Syngrapha falcifera Nuclear Polyhedrosis Virus D-clone (SfaNPV-D clone) DNA was digested with endonucleases EcoR I, Hind III, BamH I, PstI .The sizes were estimated with respect to the mobilities of standard A DNA EcoR I /Hind III fragments DNA marker. The total genome size of Sfa-D clone was about 113.12Kb.The digesting EcoR I fragments were recombined with pUCIS incubated with EcoR I in vitro and transferred into competent E.coli DH-5 a .Based on antibiotic and a -complement screening, identification of restrion.we have obtained ten hybrid plasmids inserted viral EcoR I fragments.To chose the hybrid plasmids inserted viral EcoRI fragments H in abandon and then sequenc it. Unforturnately, the result was not good. Now, the study is cont i nue.