Studies On Growth-promoting Mechanism And Residue Of Gelsemium Elegans Benth(Ge)

The study detected the mechanism of growth- promoting and residue in animal by using Ge. In the experiment, rats, mice were used to study the effect on analgesic, sedation, hypnoic by ip or im different dosage Ge total alkaloids and the effect on small intestinal peristalsis of mice by intragastricing(ig) Ge total alkaloids. After adding different level Ge powder in basic diets in 28 days, the weight gain of broilers, intestinal microflora,PH value of stomach and intestine ,activity of enzyme ,weight of ileum were measured .And at the same time, we also detected residue of Ge in broilers. The results showed as follows:1. Ge total alkaloids significantly decreased the count of wrenching abdomen reaction induced by 0.8% acetic acid in mice, and the effect increased in company with increasement of the dose. The study indicated that Ge had distinct analgesic effect.2. Irritation action of rats stimulated by haemostat inhibited and the energy metabolism of mice decreased after ip Ge total alkaloids. Ge total alkaloids also could assist the hypnotic effect of a hypnotic subthreshold dose of chloral hydrate in mice .3. Ge total alkaloids inhibited the percentage of small intestinal movel(p>0.05), but the effect increased in campany with increasement of the dose.4.After adding 1%,2% Ge powder in basic diets ,compared with the control group,the broilers' weight gain increased 5.46%,10.75% respectively.5. Ge decreased the concentration of E coli in ileum to some extent, and the decreasement related with the adding dose,i e,dose of 2% could increase obviously (p<0.05), dose of 1% have no significant effect(p>0.05), While Ge have a little influence on the concentration of lactobacillus (p>0.05),but it increased a little.And it had a little effect on the ratio of ileum weight to fresh weight(p>0.05);Ge increased pepsase activity and decreased pH value of stomach, which is related with adding dose , dose of 2% increased pepsase activity and decreased pH value of stomach obviously(P<0.05),dose of1% have a little effect(p>0.05). And Ge had a little effect on amylas activity and PH of duodenum(P>0.05).6. Ge were not found in the liver and muscle of the broilers fed the basic diet adding 2% Ge powder in 30 days and 60 days which were detected by thin-layer chromatography and gas chromatography.In conclusion ,Ge had the effect of sedation ,analgesic and little inhibited small intestinal movel ,inhibited the concentration of harmful bacterium, increased the concentration of useful bacterium .increased the secretion of gastric juice,pepsin activity, so that it could promote growth in animal.At the same time,no Ge was found in the muscle and liver of broilers.