Introduction Observation Of New Turfgrass Varieties And Establishment Of The Initiation And Regeneration System

In the first part of this thesis, 22 species of cold-season turfgrass and 6 species of warm-season turfgrass to the climate conditions of Hunan province were chosen to be introduced. In the second part of this thesis, the initiation and regeneration system for those turfgrass varieties were established. Studies on introducing of 28 turfgrass speciesEcological adaptability of 22 cold-season turfgrass varieties and 6 warm-season turfgrass varieties were evaluated. The results showed that among cold-reason turfgrass, 12 varieties are adaptive in the climate of Hunan province. For Poa L and Festuca arundinacea Schred, which is drought tolerance and no obvious domant period for the whole year, the summer survival percentage can reach 65%. For Agrostis L or Lolium L, the blade being tiny, the texture fine and smooth, the crawling character good, the summer survival rate is about 50%.As for warm-reason turfgrass, 4 varieties suitable for the climate of Hunan province were chosen. Zoysia willdand P. notatum Flugge had good resistance to cold and disease. The green period can be aslong as 245 days in the year.Establishment of the initiation and regeneration system forturfgrassThe induction rate of warm-season turfgrass is lower than that of cold-season turfgrass obviously, the highest of which being 18.75%, and mostly being lower than 5%. As for cold-season turfgrass, making use of CC or NB medium in callus inducing, the induction rate can hit to 86.25%; by getting rid of endosperm, the initiation rate of Festuca arundinacea Schred can raise by 17%. The optimal induction concentration of 2,4-D of tested turfgrass varieties is usually higher than that of rice.As far as plantlet regeneration, calli derived from warm-season turfgrass became brown and black gradually after cultured 40 days, mostly lost vigor. For cool-season turfgrass, the best 6-BA concentration for bud differentiation was 2mg/L; the best medium for differentiation and rooting of most turfgrass varieties was NB; the high concentration of 2,4-D used in the course of induction was prone to get albino plant; under the same cultural condition, the difference of rooting rate was relatively remarkable between different cultivars.