Initial Setup Of A Fast Propagation System For Rose (Rosa Rugosa Var. Plena)

Rose (Rosa rugosa van Plena) is an important ornamental plant but little has been reported about the protocol of its fast propagation. In this research, We used rose stem bearing buds as explants and studied all the factors that affected the fast propagation of rose, including time of collecting explants, sterilization, germination induction, browning controlling through shaded treatments, adventitious buds induction, shoots strengthening and roots induction. Also, we illuminated the origin of adventitious buds and adventitious roots through histological observation. Therefore, we primarily set up a fast propagation protocol of rose, which is important for improving efficiency of breeding and propagating seedlings.Our results showed that the best sterilization method is treating dormant buds with 0.5% NaCl, 0.1% HgCl2 and 0.1% Tween-20 for 5 minutes, followed by dipping into 100 mg/L gentamycin solution.In the medium of initial culture, the concentration of inorganic salts and hormone in the medium had significant effect on the germination percentage of buds induced, so did the time of collecting material. And it is optimum to collect material in April and September. Mild shaded treatment decreased the percentage of browning of rose but increased the percentage of germination. As for as controlling browning, the activity of PPO of rose shoot under natural light intensity showed no significant difference from other shaded treatments, whereas the content of phenolic substances was much higher than those treated with shaded illumination. The co-efficiency analysis demonstrated that there was no obvious correlation between the activity of phenol oxidase and content of phenolic substances.In multiplication MS medium added with 0.05 mg/L NAA and 1.0 mg/L BA, the multiplication rates was best and reached 3.44-6.00. Besides, our study also demonstrated Medium supplemented TDZ+NAA+BA has effect on the germination of rose but had little effect on multiplication of shoots when transferred from initial culture medium containing TDZ into medium without TDZ.To make shoot grow stronger, the suitable concentration of hormone was 0.3 mg/L NAA, 0.09 mg/LBA.And the percentage of shoots rooted was 41.67% in the MS medium containing 0.05 mg/L NAA and the average number of roots per shoot was 2.33.Histological observation displayed the adventitious buds and roots of rose were both originated in stele.