| The present study was carried out to evaluate the different sources' iron on laying performance and egg quality and approach to its mechanism in Roman hens.Six hundred and forty-five commercial healthy Roman laying hens of 375 days of age were randomly divided into five groups, each included three replicates of 43 layers. The layers were given a basal corn-soybean meal diet supplemented with different sources' iron for a period of ten weeks, and the control were supplemented with 80mg/kg iron (FeSO4) trial layers were supplemented with 80mg/kg iron (Gly cine-iron) for test 1, 160 rag/kg iron (Glycine-iron) for test 2, 600mg/kg iron (FeSO4) for test 3, 80mg/kg iron (nanometer FeSO4) for test 4. All birds had free access to feed and water. Five hencoops equipped with three-floored were allotted to each treatment. The dietary pretreatment was fed for one week, and the period of test in deed was 9 week, during the 9 weeks, the every treatment was supplied 16 hours light and 8 hours black in turns. During the period of feeding trial, feeding intake, death percent, the amount of eggs lay, the number of broken eggs, the total weight of eggs and the weight of eggs average were written down, and so on. After thefeeding trials, 24 eggs (three replicates which were consisted of 8 eggs each) were randomly collected, and put into 4C refrigeratory to save for analyzing. At the same time, twelve hens of each group (three replicates which were consisted of four hens each) were randomly picked out slaughter, and samples required were collected.The data of feeding trial showed that there were not significant positive effect on laying performance among the different treatments (P>0.05), but treatment groups are superior to the control; Furthermore, trial layers increased the egg's iron level compared with the control. In contrast to control, A concentration of 80mg/kg Glycine-iron and nanometer FeSO4 could raise the iron level by 6.10% (P>0.05), 43.95% (P<0.05) in yolk; 41.90% (P<0.05), 72.01% (P<0.01) in egg white. But there were not significant effect on the egg's weight average, the percent of the total egg mass conversion, and the percent of broken eggs, etc.The results of the analyse of egg quality showed that 80mg/kg (Glycine-iron) and nanometer FeSO4 could improve the egg qualities, And 80mg/kg (Glycine-iron) could increase eggshell thickness, egg specific gravity, Haugh Unit by 14.79% (P<0.05), 0.72% (P<0.01), 9.83 (P<0.05), and 80mg/kg (nanometer FeSO4) could improve the eggshell thickness, egg specific gravity, Haugh Unit by 12.31% (P<0.05), 0.54% (P<0.05), 5.05% (P<0.05) compared with control.The anaylses of the yolk's nutrition showed that the different sources' iron had not significantly effects on the percent of water, vitamin A and inosine monophosphare (P>0.05), but at 80mg/kg (Glycine-iron) could increase the level of iron and copper by 6.10% (P>0.05) and 32.11% (P<0.05) contrast to the control; 160mg/kg (Glycine-iron) could increase the contents of iron and copper in yolk by 41.79% (P<0.01) and 32.88% (P<0.05) compared with control; 600mg/kg (FeSO4) group could increase the level of iron and copper by 46.20% (P<0.01) and 25.53% (P>0.05) contrast to the control; and iron in feed at 80mg/kg (nanometer FeSOJ increased the contents of iron, copper and cholesterol by 43.95% (P<0.01), 27.66% (P>0.05) and 25.11% (P>0.05) respectively.The investigation of the egg white indicated that the different sources' iron in feed had not significant positive effects on the contents of water, copper, phosphatidic substance, inosine monophosphare. But 80mg/kg (Glycine-iron) could positive improve the content of iron by 41.90% (P<0.05); decrease the level of ether extract by 37.35% (P<0.01) in contrast to control. And 80mg/kg (nanometer FeSO4) could improve the contents of crude protein, iron and vitamin A by 8.78% (P<0.01), 72.01% (P<0.01), 13.66% (P<0.05); and decrease the level of zinc by 25.80% (P<0.05) in contrast to control.The results of hemoglobin analysis in the blood showed that each group in... |
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