Studies On The Pattern Of Organization Of Microtubules During Microspore Formation In Three Species Brassica Nepus L.

The cytoskeleton and microtubules were successively founded in animal and plant cell in 1963. The microtubule not only sustain the cells but also play important roles in the cell movements transportation and secretion of substances, transfer of cell signals, chromosome movements in mitosis and so on.To date, there are rare researches about microtubules, rarer about plant microtubules and the studied plants are mostly monocotyledon. Studying microtube-skeleton cycle of rape (dicotyledon) microspores provides evidences for the division behavior and inheritance and variation of rape microspores.The change of microtubal cytoskeleton in the processing of microspores of the restoring line Ad-6(F5), Braccica napus OguCMS and the test-crossing progenies of the restoring F5(TC1) is studied. The microtubules are dynamic and dis-assemble at low temperature in cells, so it is tied up to the research method whether the true configuration of microtube can be maintained. The enzyme digest and affixed technique of the dissociative cell is used in this experiment and it is a easy method to succeed. After little fixing the fresh tissue of gender cells and partially treating it with enzymes, strong fluorescence was labeled and the whole configuration of microtubal cytoskeleton was observed. Different conditions were adopted according to the specialty of those three anthers andgood results were obtained.The experiment results show that the change of the microtubal cytoskeleton in the processing of the microspores of the restoring line Ad-6(F5) accords to the change of general Brassicaea napus and tally in main, with the chromosome behaviors of pollen mother cells in meiosis through cell genetics methods. This ulteriorly indicates that the inheritance character of the restoring line Ad-6(F5) is steady-going. During the meiosis metaphase II of pollen mother cells in Braccica napus OguCMS, the two son-nucleus formed a spindle, but the two parts of spindle separated by the chromosome had very different sizes and the spindle didn't arrange on the same plane. The fact made it possible that the tetradproduced in meiosis couldn't develop normally to the uninucleic pollen and resulted the abnormal expressions of Braccica napus OguCMS. In the test-crossing progenies of the restoring F5(TC1), the abnormal change of microtubal cytoskeleton in the processing of microspores approved the abnormal chromosome division.