Study On Pathological Histology And Diagnosis Of Eperythrozoonsis With PPA-ELISA In Swine

Swine, naturally infected with Eperythrozoonsis,were used as experimental animals.Blood samples were collected asepsisly with the nature, and were observed by scanning electron microscope, found Eperythrozoon suis were swimming in the plasma or attaching to the membrances of red cells, which took on deformities and mastoid. The organs (heart, liver, spleen, lung, kidney and lymph node) were collected to proceed the research of pathological histology. By means of the observation of microscopy, it was found that the cardiac loop muscle fiber dyed not and all, hepatlcyte grain aberrance, there was cellulose in the spleen sinus to sink and accumulate, silt up the red cell in the lung, cystic kidney tubule epithelium cell contained necrosis; The ultrastructure results show, vacude were formed in cardiac muscle mitochondria, sarcomere contracting, the nucleosidase proteinase separated from endoplasmic reticulum of inside plasma cell (plasmacyte) of spleen sinus, the macrophages carried out their functions in liver, glomerulus basement membrane the irregularity increased thickly, the lymphoid cell pyknosis, chromatin disappeared, lung alveolar contained red cells and lysis's phagocytes.From but proof, swine infected with E.Suis will have each internal organ been damaged.SPA mark with HRP to proceed the ELISA, a rapid and sensitive method, was also established to diagnose Eperythrozoonsis, the positive serum used was prepared from rabbits immuned, the antigen was purified from infected swine, the test was responded by certain system term is: glutin 0.3% of aqueous solution, concentration of positive serum work is 1:640,and of antigen work is 1:80,the reaction time is all for 37# 30min except for the time of color appearance is 15min. Consequently, the test is specific and reliable in decting antigen of swine clinically or latently infected with E.Suis, but the specific antibody cannot be detected in the serum of naturally infected swine.The process of the operation is simple, quick, the result is easy to judge by naked eyes and it got the good specificity, sensitivity with the re-usable.