Cloning Of Gene Of Suis Eperythrozoon And Establishment Of The PCR Method For Detection Of Suis Eperythrozoonsis

The total DNA of Eperythrozoon was extracted from whole blood of an infected pig succeededly. Based on 16s rRNA sequence data , 3sets of E. suis specific primers were designed , the gene was amplified by PCR and was cloned succeededly. The objective of this study was to develop a PCR assay for identification of Eperythrozoon suis . In the meantime , specificity were examined .The results is that 936bp gene framents of Eperythrozoon suis was amplified using gene specific primers and was cloned to the pMD18-T vector. The fragment was sequenced after making restriction enzyme analyzing and PCR defined. Sequence data demonstrated that complete sequence was similarity with the published. While we identified 40blood samples using PCR, the positive rate was 90%. Proved by specificity and sensitivity experiment , PCR diagnosis was specificialy, speedly, sensitively , so it is suit for diagnosis of Eperythrozoon .The result showed , the gene cloned took material for indentification the gene constitution and expressing these gene . It was significant reference value for comprehending epidemiology and pathopoiesis mechanism. PCR diagnosis method was high specificity and sensitivity for diagnosis Eperythrozoon suis . Meanwhile , the good quality of PCR was few for antigen need, simple, quickly, precise and was suit for laboratory detection. It took a reliable diagnosis method for Eperythrozoon suis.