Wine-grape Tissue Culture And Regeneration System Establishment

Grape cuttage can not gain the virus-free plant, and it is very difficult to get the resisting diseases variety in traditional hybridization way, while tissue culture technology can finish the requirement of virus-free plant establishment and transgenic breeding, but the hinder of grape genetic engineering is that regeneration rate and transformation rate is too low.Good result was obtained by studying the tips, leaves, and petioles regeneration of Carbernet Sauvignon, Pinot Noir and Chardonnay , and studying the influence of the genotype, plant hormone, and cultural medium composition.1. By studying on the Carbernet Sauvignon, Pinot Noir and Chardonnay's shoot tips propagation, we obtained the best propagation culture medium ( Carbernet Sauvignon:MS+BAl. 5mg/L+NAA0. 02mg/L; Pinot Noir and Chardonnay: MS+BA1. 0mg/L+NAA0. 02mg/L) . By studying on the Carbernet Sauvignon, Pinot Noir and Chardonnay's propagation shoots rooting , we obtained the best rooting culture medium(Carbernet Sauvignon: 1/2MS+IBA1. 0mg/L ; Pinot Noir and Chardonnay: 1/2MS+IBA0. 5mg/L).2. By studying on the Carbernet Sauvignon, Pinot Noir and Chardonnay's leaves , petioles regeneratoion , we have obtained the leaves regeneration culture medium of Carbernet Sauvignon(MS+TDZ4.0mg/L), Chardonnay(MS+TDZ4.0mg/L), Pinot Noir (MS+TDZ6. 0mg/L+NAA0. 05mg/L) and their regeneration rates is respectively 18.1%, 6. 7%, 10.0%. We also obtained Carbernet Sauvignon petioles regeneration culture medium(MS+TDZ4.0mg/L+NAA0.01mg/L) and it's regeneration rate is 28.6%, and obtained the rooting medium of Carbernet leaves regeneration buds (1/2MS+IBA1.0mg/L).3. The research indicates that the cytokinin BA have no effect on the leaves regeneration of Carbernet Sauvignon, Pinot Noir , and the KT have no effect on the leaves regeneration of Carbernet Sauvignon, Chardonnay. Increasing the cytokinin concentration and kinds do not raise the regeneration rate of the leaves in vitro, and also find that selection of explant is very important to the explant regeneration, and regeneration rate of leaves with petiole stock is higher than the leaves without petiole stock.