Studies On The Technique System Of Tissue Culture And Mutation With Colchicine In 'Red Globe'Grape (Vitis Vinifera L.'Red Globe')

The young stem segments of Red Globe grape were used for explants in the study on the technique system of micropropagation and mutation with colchicine. Firstly, the micropropagation procedures of Red Globe grape was investigate; including the establishment of initiation culture, shoot proliferation, rooting and acclimatization, and the optimal medium and culture program were studied. Secondly, colchicine was used for inducing plantlets of Red Globe grape, some of the induced plantlets were analyzed at the molecular level with random amplified polymorphic DNA(RAPD). Finally, different transplant media were compared in transplanting phase. The results were as follows.1) Initiation culture(1)B5 basal medium was the most suitable initiation culture basal medium.,(2)The optimal culture media for initiation culture were B5+0.5mg/L6-BA+0.1mg/LNAAand B5+1.0mg/L6-BA +0.2mg/LNAA (3)6-BA was the best cytokinin which could facilitate lateral bud sprouting. ZT took secondplace, KT was not satisfactory.2) Shoot proliferation(1) The optimal culture medium was B5+0.05mg/L6-BA+0.2mg/LIBA.(2) The proliferation couldn't be promoted by adding peach juice or grape juice in the medium.(3) Glucose was better than sucrose .3) Strengthening and rooting medium was B5+0.1 mg/LIBA+0.2%AC.4) Experimentation of RAPD(1) The improved CTAB method was the best one for the extraction vitis genomic DNA. DNA could only be extracted from young leaves of the plantlets in vitro. DNA from young and tender leaves had better purity and the output was high. Though the output...