Research On Application And Antiviral Mechanism Of WCT-â…¡ Extracted From The Plant

WCT- II is a kind of antiviral material, which is withdraws from the plant by extraction and the heavy crystalloid . In this study , we analyzed its physical and chemic properties. We take tobacco mosaic virus and tobacco , watermelon mosaic virus-2 and vegetable marrow as objects , measure control effects of WCT- II indoors and research on antiviral physiological-biochemistry mechanism of WCT-II.WCT- II is a kind of orange crystal, can dissolve in the acetone , ethyl ether and n-butyl alcohol ,tiny dissolve in the chloroform and alcohol, can't dissolve in the water and petroleum ether . By IR , MR and HMR , WCT- II is lanost-8-en-oic , 3-alpha , 12-alpha-dihydroxyl-24-methylene-ethylester.WCT- II can control virus diseases by biological control effect. The efficiency is 73.6 % , when WCT- II is sprayed on tobacco at 120ppm ;The efficiency is 52.6% , when WCT - II is sprayed on vegetable marrow at 1 OOppm . After treated by WCT- II, virus pathogen-icity obviously decrease by withered spot half leaf's method and virons break up in large number by electron microscopy .The plant occur a series of physiological-biochemistry changes by WCT- II. Compare with check , chlorophyll's quantities obviously increase , mosaic symptom alleviate . Cata-lase activity is obviously inhibited . Chitinase activity and beta- 1, 3 - glucanase activity in leaves and intercellular compared with control are obviously increased ; the proteins existed in leaves and intercellular is analyzed through SDS-PAGE , the result show that a specific acid-soluble protein during the 5th-9th day and a specific basic-soluble protein in the 7th day exist in tobacco's intercellular, a specific acid-soluble protein exist during the 3rd-9th day in vegetable marrow's leaves .WCT- II is a kind of antiviral material from plants . It has some antiviral mechanisms , which can primarily blunt virus outside and induce the host resistance (increase quantities of the chlorophyll, repressed catalase activity , increase chitinase activity and P - 1, 3 -glucanase activity , induce the PRs ). High biological activity WCT- II is nontoxic and safe to animals and plants . It will be applied extensively in the future .