| The study was conducted to examine the effect of intrauterine growth restriction (IUGR) on pancreas and stomach using a model of neonatal pigs. Fourteen pairs of pigs, where each pair consisted of one pig of normal body weight (NW) and one littermate with IUGR (IUGR), were employed and divided into 3 groups that were newborn group (Newborn), 3-day-old group (D3) and 7-day-old group (D7). The study comprised two experiments.The first experiment studied the effect of IUGR on pancreas in neonatal pigs. Pancreas weights, contents of protein, DNA and RNA of pancreatic tissue, histological and morphological parameters, activities of pancreatic digestive enzymes and contents of insulin and glucagon were examined in the first experiment. The result showed that pancreas weights of both IUGR and NW pigs significantly increased from birth to 7Ul day (p<0.01) with slower speed in IUGR pigs. Compared with the NW pigs, Pancreas weights of IUGR pigs of Newborn, D3 and D7 all significantly decreased (p<0.01) with respective reduction of 28%, 28% and 30%. Contents of protein, DNA and RNA in pancreatic tissue of IUGR pigs of Newborn, D3 and D7 also significantly decreased (p<0.01 or p<0.05). Mean acinar size and acinar cell size of all groups significantly decreased (p<0.01).From birth of 7th day, total activities of trypsin, chymotrypsin, amylase and lipase of both IUGR and NW pigs all increased while activities of enzymes of IUGR pigs increased at a slower speed. Compared with the NW pigs, total activities of trypsin of IUGR pigs of Newborn, D3 and D7 respectively decreased 21%, 24% and 43% (p<0.05). And total activities of chymotrypsin respectively decreased 33%, 28% and 36% (p<0.05). Total activities of amylaseof IUGR pigs of Newborn and D3 did not show significant differences to NW pigs whereas the activities of IUGR pigs of D7 significantly decreased 37% (p<0.05). Total activities of lipase of IUGR pigs of Newborn and D3 were all significantly reduced (p<0.05) but the activities of IUGR pigs of D7 did not show significant difference.From birth of 7th day, contents of insulin of both IUGR and NW pigs increased however contents of glucagon decreased. Compared with the NW pigs, total contents of insulin of IUGR pigs of Newborn and D7 tended to decrease significantly as indicated by respective reduction of 22% and 37% whereas the parameter of IUGR pigs of D3 did not show significant difference. Total contents of glucagon of Newborn, D3 and D7 respectively decreased 33%, 33% and 36% and tended to decrease significantly.The second experiment studied the effect of IUGR on stomach in neonatal pigs. Weights and contents of protein, DNA and RNA of gastric tissue, histological and morphological parameters and total milk-clotting and proteolytic activity of gastric protease were examine in the second experiment.The result show that weights and contents of protein, DNA and RNA of gastric tissue, thickness of stomach wall of fundic region all increased from birth of 7th days. Compared with the NW pigs, the stomach weights of IUGR pigs of all groups all significantly decreased (p<0.01); Total contents of protein, DNA and RNA of IUGR pigs of all groups also decreased significantly (p<0.01 or p<0.05); Thickness of stomach wall of fundic region of IUGR pigs of all groups significantly decreased (p<0.05).From birth of 7th day, total milk-clotting and proteolytic activities of gastric proteases all increased. Compared with NW pigs, total milk-clotting activities of gastric protease of IUGR pigs of Newborn, D3 and D7 respectively decreased 38%, 58% and 29% as tended to decrease significantly; Total proteolytic activities of gastric protease of IUGR pigs of Newborn, D3 and D7 respectively decreased 41%, 23% and 46% as tended to decrease significantly.It is concluded that, compared with NW pigs, weights of pancreas and gastric tissue, contents of protein, DNA and RNA, some pancreatic and gastric histological parameters and total activities of digestive enzymes of IUGR neonatal pigs were significantly decreased although weight...
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