| The role of daidzein, HK02,F89,F89 II in control of the feed intake and laying performance in hens and involved endocrine mechanisms were studied in this research, the present research include 4 series:Series 1: Effect of diet supplemented with daidzein and F89 in long-term on the feed intake and laying performance in laying hens and endocrine mechanisms involved.60 26-week-old hens were randomized into 3 groups : basal diet, basal diet+6mg/kg daidzein , basal diet +2mg/kg F89 . Compared to control , the feed intake of basal diet +daidzein group was increased, and laying performance was improved ; serum 17β - estradiol, T3, T4 levels were increased whereas the serum leptin level was reduced ; The feed intake of basal diet +F89 group was increased and laying performance was also improved. Serum insulin and leptin levels were increased.Series 2: Effect of diet supplemented with HK02 and F89 II on the feed intake and laying performance in older laying hens and endocrine mechanisms involved.60 49-week-old hens were randomized into 3 groups : basal diet, basal diet+6mg/kg HK02 , basal diet +2mg/kg F89II . Compared to control , the feedintake of basal diet +HK02 group was increased, and laying performance was improved ; serum 17 β- estradiol, T3, T4 levels were increased whereas the leptin level was reduced ; The feed intake of basal diet + F89II group was increased and laying performance was also improved. Serum insulin and leptin level were increased.Series 3: Effect of diet supplemented with daidzein and F89II on the feed intake and laying performance in older laying hens and endocrine mechanisms involved in the condition of high environmental temperature.60 53-week-old hens were randomized into 3 groups : basal diet, basal diet+6mg/kg daidzein , basal diet +2mg/kg F89II . Compared to control , the feed intake of basal diet +daidzein group was increased, and laying performance was improved; serum T3, T4 levels were increased whereas the leptin levels was reduced; The feed intake of basal diet + F89II group was increased and laying performance was decreased . Serum T3, T4, leptin levels were increased.Series 4: Effect of diet supplemented with daidzein on body SOD and MDA level in older laying hens40 60-week-old hens were randomized into 2 groups : basal diet, basal diet+6mg/kg daidzein , Compared to control, the feed intake and laying performance of basal diet +daidzein group were increased, serum SOD and liver SOD levels were increased whereas the hypothalamus SOD levels was reduced ; Serum MDA, liver MDA, hypothalamus MDA levels were all reduced.In conclusion, daidzein (6mg/kg diet) can improve the performance and regulate concentrations of some blood hormones in laying hens and it can also perform the effect of anti-oxidation in older laying hens. It seems that the effect of daidzein may be partly related to the alteration of blood 17 P - estradiol, T3, T4, leptin , etc; F89II also increase feed intake and performance hi laying hens, the effect of F89II seems partly related to the alteration of blood insulin, leptin, etc. |