| In the research, we have modified the Food Intake Data Acquisition System (FIDAS), using the materials made in our country. Then we studied the effect of adding 50mg Y -Aminobutyric acid(GABA) and/or 2mg Flurazepam (F89) in every kilogram diet on feed intake in young laying hens and endocrine mechanisms involved. We hope that we could find a way to make GABA serve in stockbreeding. We also discussed the mechanisms of F89 in increasing feed intake of animals. The research included 4 parts: Part one: Modification of the FIDAS .We applied the Medlab system of Nanjing Medease Science and Technology CO. LTD to modify the FIDAS system. After modified, the system was not only easy to maintain, but also capable of stronger ability in data collection, storage and analysis, and it was easy to operate. We used this system studied the feed intake pattern of young laying hens, which proved the modified FIDAS was fit for the study on feed intake behavior of animals.Part two: Effect of diet supplemented with GABA and/or F89 on feed intake in young laying hens.This part was divided into two series. Series I : Four young laying hens (two hundred and ten days old) were used. The experiment was divided into three periods: control period, the animals were fed basal diet; GABA period, basal diet supplemented with 50mg/kg GABA;F89+GABA period, basal diet supplemented with 2mg/kg F89 and 50mg/kg GABA. The modified FIDAS was used to observe the feed intake pattern. Compared to control period, GABA have relatively small effect on feed intake of young laying hens; the F89 and GABA together have stronger effect on the feed intake of these animals, which made the feed intake (15. 76%, P<0. 05)and the number of meal(12. 56%, P<0. 01)increased and the time of feeding reduced (15. 12%). The two experiments periods also had strong difference, we presumed thatthe F89 play a main role in the course.Series II:Four young laying hens (two hundred and thirty days old)were used. The experiment was divided into three periods: control period, the animals were fed basal diet;F89 periods, basal diet supplemented with 2mg/kg F89;F89+Pic periods, basal diet supplemented with 2mg/kg F89 and infusion of 1.25mg/kg picrotoxin (Pic)in the wing before adding food. The modified FIDAS was used to observe the feed intake pattern. Compared to control period, F89 increased the feed intake (10. 34%, P<0. 05)and the number of meal (8. 37%, P<0. 01) and reduced the time of feeding (12. 37%). After infusion the Pic, the action of F89 on feed intake was prohibited in a degree, and the change of feed pattern in young laying hens bring by F89 was reduced correspondly. This series suggested that the F89 can via the GABAA receptor to increase feed intake.Part three: Effect of diet supplemented with GABA and/or F89 on serum hormones in young laying hens.This part was divided into three series. Series I :The sample were get from the young laying hens in series I of part two, and the grouping was same. The RIA data showed: Compared to control period, 50mg/kg GABA increased the concentrition of serum hormones, but the effect was relatively small; the F89 and GABA together also increased the concentrition of NPY (11.04%), insulin (23.03%, P<0.05), glucagon(7. 50%, P<0. 05), T3 (19. 75%, P<0. 05) and T4 (13.45%).Series II :The sample were get from the young laying hens in series II of part two, and the grouping was same. The RIA data showed: Compared to control period, the concentrition of NPY(9. 87%), insulin (19.24%, P<0. 05), glucagon (5.15%), T3 ( 16.46%, P<0.05) and T4 (14.73%)all increased in the F89 period. After the infusion of Pic, the concentrition of these serum hormones reduced, but still higher than the control's.Series III: Eighteen young laying hens (two hundred and ten days old) were used in this series. The experiment was divided into three groups:control group, the animals were fed basal diet;GABA group, basal diet supplemented with 50mg/kg GABA;F89 group, basal diet supplemented with 2mg/kg F89. There are six animals inevery group. T...
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