Phylogenetic Study Of Three Isolates Of Anaplasma Ovis In China Based On 16S RRNA Gene

Anaplasma ovis is an arthropod-borne rickettsial obligate parasite of sheep and goat. The disease caused by A. ovis is widespread in the northwest of China and has become latent threatening to development of sheep husbandry. Traditional criteria for identification Anaplasma species have relied on host pathogenicity and the location of inclusion bodies within host red cells. It's obvious that those methods have certain shortcomings. In this study, DNA was extracted from three A. ovis isolates which came from Jingtai, Yuzhong and Zhangjiachuan counties in China respectively and amplified with primers designed in light with 16S rDNA; and amplicons were ligated with pGEM-T vector, then transformed to E. coli JM109 and selected positive cloning to multiply and sequencing. These sequences were compared with available GenBank accessions' sequences of 7 A. marginale isolates, 2 A. centrale isolates and 3 A. ovis isolates which from America, Uruguay, South Africa, Zimbabwe, Australia and Israel and inferred phylogenetic tree. It indicates the lowest homogenic percentage between foreign three A. ovis and this study's is 99.3%. Majority of erythrocytic Anaplasma species were classified to three different clusters from the phylogenetic tree. It demonstrates all Anaplasma species have closely relationship and the 16S rRNA gene analyses are useful to define Anaplasma species with phenotypic and traditional taxonomies.