The Phylogenetic Relation Of Ovine Babesia Sp Based On 18S RRNA Gene Sequencing

The phylogenetic relation of Ovine Babesia sp in Hebei, Liaoning province and at Lintan, Ningxian in Gansu province China, were analyzed by using 18srRNA sequence. The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector. It was identified by restriction endonuclease analysis and PCR and sequencing. The results suggested that the length of 18s rRNA gene four Ovine Babesia sp involved in this study was around 1800bp. Two phylogenetic trees was inferred based on 18srRNA sequence of Hebei, Liaoning province, Lintan, Ningxian in Gansu and the other species of Babesia and Theileria available in GenBank.In the first tree, it was analyzed that the homologies of species of Hebei and Liaoning, Lintan and Ningxian are 95.8%, 91.4%, respectively. In the second tree, it is indicated that the species isolated from Lintan, Ningxian, Liaoning located between B.divergens and B.bigemina, however, the species from Hebei were between B.bigemina and B.caballi.