| The alkaline phosphatase (AKP) exists in various kinds of organism extensively.Now AKP has been isolated from various kinds of organism such as bacterium, germ, alga, higher plant, insect, aquatic animal, mammal, people,etc. and applied to the daily life through many ways, such as medical science, toxicology, nucleic acid research, measuring of immunology, food, cosmetics, morbib examination of persons, animals and plants. At present,many aspects of AKP have been reported. By way of medicine there are AKP products in Korea S., Japan, and some other countries but few in China.Moreover,the technology of isolation and purification of AKP is more complicated, and be limited by enzyme source at the same time , so it seems particularly important to develop new AKP source.Bombyx mori is both an important economic insect and also an important model organism for the molecular genetics study. AKP is as a kind of functional enzyme in silkworm's body , closely related to the digesting and assimilating, metabolism and transportation of matter, its economic properties and physiological state.lt has already caused the domestic and international scholar's extensive research , but present domestic research concentrates on its physics and chemistry nature, active influence factor.etc. on the basic theoretical research mainly, and investigate to its activity on a large scale and genetic way, has not seen the relevant report. Though there have some reports about some physics and chemistry properties, dynamics character of AKP .separated from silkworm midgut abroad, and has cloned the whole gene of AKP, but up till to now, we have not found yet the research report about application of silkworm AKP directly. In this investigation, through investigating intestinal juice AKP activity of 84 silkworm variety and heredity system from the Southwest Agricultural University silkworm gene library, we find some silkworm varieties havehigh AKP activity extremely, and this silkworm AKP probably directly used in large-scale production.So purification of the AKP from this variety ,to study its nature and characteristic has important research value.Thinking bases on this, we first select variety with high and low AKP activity to cross.According to the investigation result, we have analysed its hereditary law , then isolate the silkworm's intestinal juice AKP, to carry on research on its basal physics and chemistry nature and functional characterization.The bloods relationship of the silkworm and wild silkworm are on intimate terms. As an abundant wild sericultural resource, wild silkworm is a treasure with much potential to exploit. And also be used as an important material for the study on the origin of silkworm. So far extensive study has carride on to wild silkworm, in biology study ,structure and variation research of chromosome,, DNA polymorphism study, in silkworm application study on breeding and prevention and cure research as the pest of (he mulberry field. But for its thin cocoon layer, short silk, though the study on wild silkworm has already relatively been deepened at present, but is still limited to a certainty. In recent years, with the development of molecular biology, the molecular system about wild silkworm and silkworm make certain progress too, research at the gene level launch in succession, such as silk albumen gene, RNA of ribose gene research,etc., but not find the report about the research of the wild silkworm AKP gene. So,in this invetigation.with wild silkworm as material, according to the whole gene sequence of silkworm AKP .already announced in GenBank, follow the design principle of peculiar primers , design the corresponding primer, we have cloned part sequence of the wild silkworm AKP gene.With its deduced amino acid, cDNA, DNA sequence correspond with the silkworm and other species sequence,to make comparative analysis, thus analyse their relation and evolutionary mechanism.Now report the results of study as follows: 1. Activity Investigation and Genetic AnalysisThrough AKP activity investigatio...
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