Analysis Of Random Amplified Polymorphic DNA Of Genetic Diversity And Relationship Of Five Species Of Palaemoninae

In this paper,random amplified polymorphic DNA (RAPD)technique,together with the method of morphology,was employed in analyzing the genetic diversity and the genetic relationship among five species of important Palaemoninae prawns.In this experiment,33 out of 40 random primers (10 bp) were selected to carry out the analysis of RAPD and 304 DNA fragments were amplified among two species of Genus Coutierella.The number of DNA fragments amplified of Coutierella tonkinensis tonkinensis and Coutierella tontdnensis guangdongensis was 279 and 296, respectively,and 184 and 191 of them were polymorphic,respectively,amounted to 65.9% and 64.5% respectively.The genetic diversity index was 0.163 and 0.129, respectively. Interpopulation and intrapopulation average genetic variation of two species Coutierella tonkinensis was 0.942 and 0.058,respectively.There was no RAPD marker amplified by 33 primers. The cluster analysis of the above two species was performed with UPGMA algorith by using DPS(3.11 professional version)software package.The result is not as same as the result of morphologic analysis.The genetic similarity index and the genetic distance among the two species of Coutierella tonkinensis prawns was 0.943 and 0.057,respectively.31 out of 40 random primers(10 bp) were selected to carry out the analysis of RAPD and 450 DNA fragments were amplified among three species of Genus Macrobrachium.The number of DNA fragments amplified of M.inflatum, M.hainanense and M.formosense was 353,316 and 270,respectively,and 228,147 and 61 of them were polymorphic,respectively,amounted to 64.6%,46.5% and 22.6%,respectively.The genetic diversity index was 0.211,0.135 and 0.142,respectively.Interpopulation and intrapopulation average genetic variation of the three species was 0.682 and 0.318,respectively.33 amplified fragments were distinctive among the three species with the molecular weight varying from 259 bp to 3333 bp. The cluster analysis of the above three species was performed with UPGMA algorith by using DPS(3.11 professional version)software package. The individuals of the same species clustered together before the different species did.The result was as same as the result of morphologic analysis. The genetic similarity index among M.hainanense and M.formosense,M.hainanense and M.inflatum, M.formosense and M.inflatum was 0.845,0.694 and 0.706,respectively.The genetic distance of them was 0.155,0.306 and 0.294,respectively...