Study On Genetic Diversity Of Pennisetum By RAPD And ISSR Marker

45 Pennisetum Rich materials from Fuzhou and Jianyang were studied for optimizing the RAPD and ISSR's reaction conditions and procedure. Pennisetum Rich's genetic diversity and relationship were studied by RAPD and ISSR markes.The major results showed as follows:1.Through the orthogonal design choosed the experimental procedure and optimize the proxedure. We found the obtained optimum scheme on reaction system for RAPD-PCR:1.5μLMg2+(25mM),0.4μL dNTPs(10mM),1.2μLprimer(10pM),0.4μLTaq DNA Polymerase(5U/μL),2.0μL 10×PCR Buffer,2.0μL DNA,ddH2O 12.5μL;ISSR-PCR:1.5μL Mg2+(25mM),0.3μL dNTPs(10mM),1.5μL primer(10pM),0.4μL Taq DNA Polymerase(5U/μL),2.5μL 10×PCR Buffer,2.0μL DNA,16.8μL ddH2O.2.The analysis of RAPD marker showed that eight RAPD primers amplified a total of 70 scorable markers,percentage of polymorphic loci was 88.57%,Nei's genetic diversity and shannon's information index were 0.3080 and 0.4541,respectively.The analysis of ISSR marker showed that nine ISSR primers amplified a total of 83 scorable markers,percentageof polymorphic loci was 92.77%,Nei's genetic diversity and shannon's information index were 0.2832 and 0.4280,respectively.The results of RAPD and ISSR showed that the genetic diversity of forage resources on pennisetum Rich was enrich.3.The matrixes of genetic distance was established on RAPD and ISSR fingerprint,respectively.The results of UPGMA cluster analysis based on RAPD and ISSR fingerprint had the approximate trend,but not the same.