| Chinese chive was originally cultivated in China, It has a long history and a great variety of cultivation. Because of plentiful value of nutrition and medicine, Chinese chive has been one of important vegetable in north China. The people have done some research to Chinese chive in cultivation technique, the regularity of morphdoyical development , physiological characteristics since the 1950s, However, up to now the report as to nutritional absorption of nitrogen and nitrogen-regard law have not appeared. Cultivar Chinese chive was researched in the effects of nitrogen concentration forms and proportion on growing physiological character the quality of nutrition and key Enzyme in nitrogen metabolism for providing theoretical basis of Chinese chive manure rationally and soilless culture.1. The effects of nitrogen concentrations on growth, nitrate content and the quality of Han zhong Chinese chive (Allium tuberosum Rottler) in hydroponics was tested. The results showed that when the nitrogen concentration was in the range of 2-20 mmol-L'1, the leaves grew faster of in the ten days after cutting, then the leaves grew slower. When the nitrogen concentration was in the range of 8~12 mmol-L"1, the yield, the content of Vc, soluble sugar and soluble protein were at higher level. The content of nitrate was positive correlation with the activity of nitratase. The suitable content of nitrogen is 8-12 mmol-L'1 in Hydroponics with Chinese chive.2. In the nitrogen concentration of 8 mmol-L"1, cultivar 791 and Hanzhong Chinese chive were studieded in the effects of different nitrogen forms and proportion on growth, nitrate content and the quality of nutrition. The result showed that with the increase of NH4+ and the drop of NO3", their fresh weight, dry weight and the yield of the top and root rose at the beglinning, the decline; firstly and dropped cater ; the change of soluble protein had the similar trend ; However, the nitrate and Vc content of the leaf and nitrate reductase activity gradually declined ; the suitable rate of soluble sugar total free amino acids and cellulose gradually rose. According to comprehensive measure index of the yield and quality of nutrition, the suitable rate of NO3-: NH4+ was 2:2 or 1:3 for 791 and 3:1or 2:2 for Hanzhong Chinese chive.3. The effects of different nitrogen concentration, forms and proportion on the root activity, chlorophyll and mineral elements of Chinese chive. The result showed that when the nitrogen concentration was in the range of 8-16 mmol L-1 and the proportion of NO3-: NH/ in the range of 3:1-1:3 the root activity of Chinese chive chlorophyll and absorption of mineral elements studied was N P K were all enhanced.4. The effects of the different nitrogen forms and proportion on the GS, GOT and GPT in the nitrogen metabolism on cultivar Hanzhong Chinese chive. The results showedthat when the rate of NO3-:NH4+<1 in the nutritional liquid, with the increase of NH4+ GS activity in roots and leaves was strengthened ,peaked when the rate of NO3: NH4+ was 2:2 , then declined. At the same time, with the increase of NH4+ in the nutrition liquid, the change of GOT and GPT activity in roots rose at first , then drop later. However , in the leaves , the GOT activity was rarely influenced by the nitrogen forms and GTP activity decrease .5. This experiment make the suitable nitrate concentration and proportion of NO3-:NH4+ clear in Chinese chive, providing theoretical basis for rational fertilization and soiiess culture. By testing the contents of several key enzyme under different nitrogen concentration , forms and proportion, we concluded that the higher NH4+ concentration in the nutrition liquid can enhance the activity of GS, promoting the nitrogen metabolism.
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