The Study Of The Processing Technology Of Biologic Peptide Of Soybean Meal And Its Effect On The Performance And Blood Indicator Of Broilers

In this test, the production method of using bacterium fermenting soybean meal through selecting strain, optimizing the condition in the spinner flask, culturing in the fermenter and analysing the character of the ferment production was studied. And the effect of the production on the broilers was studied. 320 1-day-old Avain broilers were randomly allotted into five groups with 4 replicates of 15 birds each replicate. Group 1 was the basic group, fed basic diet, the other four groups were fed with basic diets adding 0.1%, 0.5%, 1.0%, 1.5% the biologic peptide of soybean meal respectively, studying the effect on the production performance, serum, immunity and the caecum microorganism of broilers.The processing technology of the biologic peptide of soybean meal was determined as follows: (1) bacterium fj625 was the fermented strain; (2) the optimum conditions in the spinner flask were: soybean meal 15%, glucose 1%~4%, MgCl2 0.02%, K2HPO3 0.01%, pH 6-7, granularity of the soybean meal < 40 holes; (3) the fermentation medium of the fermentater contained: soybean meal 15%, glucose 1.5%, MgCl2 0.02%, K2HPO3 0.01%; the fermentated volume occupied by 50% of fermenter cubage; natural pH; inoculation volume 5%. (4) during the period of fermentation, the temperature was 30C, controlled the density of oxygen by controlling the agitation speed and ventilate; maintained the pressure at 0.5Mpa.The nutrition level of the biologic peptide of soybean meal were: CP 49.27%, TE 16.64MJ/kg, EE 3.02%, CF 11.84%, Ash 8.6%, Ca 0.38%, TP 0.87%, NFE 15.79%. The molecular weight of the biologic peptide of soybean meal were all smaller 8000Da, the ratio of the peptides from the soybean protein was 42.01%, neutral proteinase activity was 1538?5.26U/g.The result of animal experiment showed that: (1) the average daily gain of the experiment groups increased with the increasing of adding level of the biologic peptide of soybean meal. It was significant difference between the group 5 (adding 1.5%) and the group l(p<0.01). In the whole period (0~6w), comparing to group 1, the average daily gain of the group 2, 3, 4, 5 were increased by 3.03%, 3.52%, 4.60%, 7.40% (2) In the whole period (0~6w), comparing to group 1, the average daily feed intake of the group 2, 3, 4, 5 were increased by 4.75%, 4.54% 3.33% 3.06%. The group 4 and group5 could increasethe average daily feed intake significantly (p<0.05). With the increasing level of the biologic peptide of soybean meal, the average daily feed intake was in the downward. (3) In the prephase period (0-3w), comparing to control group, adding 1.5% biologic peptide of soybean meal to broiler diets decreased F/G by 7.36%(p<0.05). (4) In the prephase period (0~3w), the diets containing 1.0%, 1.5% biologic peptide of soybean meal saved the feed cost 0.09 yuan/kg and 0.15 yuan/kg respectively. In the anaphase period (4~6w), the diets containing 1.5% biologic peptide of soybean meal saved the feed cost 0.05 yuan/kg. (5) It was no significant difference of the serum protein and serum urea nitrogen of the 21-day broilers (p>0.05). Comparing to the control group, the TP of the 42-day broiler's serum of the experiment groups were increased and there was significant difference between group 4 and the control group (p<0.05). There were no significant differences of the globin among the experiment groups (p>0.05). The content of the albumin of the experiment groups adding the biologic peptide of soybean meal were higher than control group. It was significant difference between the group 3 and the control group (p<0.05). Group 4 and Group 5 could increase the albumin significantly (p<0.01). The serum urea nitrogen was not significant changed (p>.05). (6) There were no significant differences of the immunity organs index by adding the biologic peptide of soybean meal. Increasing the spleen index of the 42-day broiler. It was significant difference between the group 3 and the group 1 (p<0.01) and it was significant difference between the group 4 and the groupl (p<0.05). The trend was same as the 21-day's. There w...