| It's one of the most desiderate difficulties to select high quality bait-algae and that be well cultivated to spat cultivation of Coelomactra antiquate. The paper studies Coelomactra antiquate, s decelicous bait-algae in three development stages and Diorateria XR-03-02 ,s bait value and the fcasibility on stream which separated form the sea area atchangle city, traverse about Diorateria XR-03-02 is the physiological and ecological characteristic ,main nutrient content , and the best culture medium. Part of physiological characteristic and systematic status of Skeletonema XR-03-06 are also studied at the same time. The mian results are follows:1. The breed effects of 12 bait-microlage which have been in common use and three wild algae on Coelomactra antiquate larva are compared. . Result is : Diorateria XR-03-02 , D. zhanjiangensis Chlorella sp and C. marine are the dainty bait for Coelomactra antiquate larva with vclum. Platymonas subcordiformisn. and Pyramimonas sp.fit Coelomactra antiqiiatalarva with umbo. Chaetoceros mulleri and Skeletonema XR-03-02 fit Coelomactra antiquate post-larva.2. Micromorphogical and ultrastructurocharacters of Diorateria XR-03-02 are examined by LM SEM and TEM. absorption spectrum of Diorateria XR-03-02 culture is scaned by spectrophotometer . pigment extraction is separated by TLC(thin-layer chromatography) with homemade iicagel . Results: Diorateria XR-03-02 is nakedness and free unicellular. 11, s (5?) X (2?) urn in size, sub-cycloidal or long-elliptical in shape. Vegetative cell of Diorateria XR-03-02 has an isometric whiplash type flagellum which is acrogenous and a big cyathiform chromatophore which occupy the whole cell nearly . the culture ofDiorateria XR-03-02 has maximum absorbency in 440 nm and 470 nm so the carotinoid is the main pigment can be estimated. Analysis shows the pigment extraction includes chlorophyll a chlorophyll c,B-carotene fucoxanthin diadinoxanthin, diatoxanthin and lutein.All above and referring to literature we conclude systematic status of Diorateria XR-03-02 is chrysophyta, Chrysophyta, Chrysomonales, Isochrysidaceae , Diorateria.3. The physiological and ecological characteristic of Diorateria XR-03-02 is studied by analysis of cell density , biomass , growth rate, chlorophyll, photosynthesis oxygen evolution rate and nitrataseactivity. Results show: The growth rate of Diorateria XR-03-02 is near to Isochrysis sphacrica, Diorateria. zhanjiangensis and fsochrysis galbana 3011, s. but it has characteristic such as a longer exponential phase , and higest cell density 1.7X107/m.l . Diorateria XR-03-02, s photosynthesis oxygen evolution rate exceed the other two in the low light intensity .and it,s nitratase activity is also better than the other two . Diorateria XR-03-02 has a wide range of ecologic adaptation : moderate temperature 15-35C , optimal temperature 25C ; proper light density 1000-10000Lux , 5000-8000Lux is optimum; alkalinity acidity accommodation limit pH 4-11, pH 7-9 is optimum ; wide salinity range 5%-60%, 20-33%, is optimum.4. The nutrition components of Diorateria XR-03-02 are studued by chemical methods . Protein mensurated by Kjeldahl determination. Raw fat extracted by extractor, aminoacid anal ysed by automatic ami no acid analyzer and fatty acid analysed by GC (gas chromatography). The compostions are :crude protein 36%. DW, total amino acid 28.07% DW, essential amino acid 51. 6% total amino acid, crude fatty acid 43. 5% DW. The content of saturated fatty acid is : nutmeg, palmitic acid and stearic acid, unsaturated fatty acid :palmitoleic acid,oleic acid, linoleic acid and linolenic acid. The results show that the major components of unsaturated fatty acid, about 72.3% total fatty acid .especially linolenic acid 43.96% including r-Linolenic acid 37.44%.5.The optimal producibility medium culture of Diorateria XR-03-02 is CO (NH)2-20 mg /U NaNO3-35 mg/U KH2P04-3. 75 mg/K, FeC6H507-2. 5mg/U B1-100ug /L B12-5 ug/L, which has been selected from five prescriptions and optimized by quadrature analysis according t...
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