A Study On The Variation And Its Exploitation By Transferring Exogenous DNA Into Maize Inbred Lines

The exogenous DNA from different plants was introduced into the different maize inbred lines through seed-immersion method and pollen-tube pathway method. After surveying the quality of field growth and counting the number of chromosome of cell and analyzing the zymograms of peroxidase isozyme (POD) and the structure of leaf.The result is as follows:1.The seed-immersion method:(1) The total DNA from maize inbred lines 12-9-10 , Bangladesh super sweet maize inbred lines and sugarcane was introduced into different maize inbred lines with three densities of 300 g/ml,500 g/ml and 700 g/ml separately. The best density of exogenous DNA introduced is 300 g/ ml.(2) The mutant rate of D0 generation of the combination of maize inbred lines 12-9-10 sugarcane(DNA)is 2.22%. After introducing the sugarcane DNA with the density of 300 g/ml into maize inbred lines 12-9-10 ,a opposite leaf plant D0waxyx sugarcane 300) was obtained from D0 generation .Its leaf structure was inclined to the donor and a peroxidase -isozyme strip which was of donor and not of receptor appeared in Rf5= 0.3 3and Rf6=0.40 of peroxidase isozymogram.and a new peroxidase -isozyme strip which was of neither donornor receptor appeared in Rf8 =0.61 of peroxidase isozymogram. A mutant plant which has not opposit leaf appears in DI generation and it was short, precocious, thick green color in leaf. Opposite leaf plants appeared in D2 generation .The opposite leaf plants in proportion to the mutual leaf plant is 112:53.(3)The mutant rate of D0 generation from the combination of maize inbred lines 12-9-10xBangladesh super sweet maize lines(DNA)is 11.76% and 5.88%. With the density of 300 g/ml and 500 g/ml separately ,two mutant plants D0 -2and one mutant plant D0(waxyxmongt500) were obtained separately. A peroxidase -isozyme strip which was of donor and not in receptor appeared in Rf6=0.59 of peroxidase isozymogram of the mutant plant D0 (waxy mongooo) -2 . The peroxidase isozymograms of the other two plants were not mutant .Three mutant plants appeared in D1 generation .(4)With the density of300 u g/ml ,the combination of super sweet maize inbred lines 5-48 X maize inbred lines 12-9-10(DNA) acquired one mutant plant in DO generation and acquired one mutant plant in DI generation. A peroxidase-isozyme strip which was of donor and not of receptor appeared in Rf6=0.62.The rest plants were not mutant.2.Pollen-tube pathway method(l)17h,19h,21h and 23h after pollination ,the total DNA from maize inbred lines 12-9-10 and Bangladesh super sweet maize inbred lines separately was introduced into Bangladesh super sweet maize inbred lines and maize inbred lines 12-9-10 with the 500 g/ml density of DNA. The result indicated that: thehighest mutant rate in DI generation from the combination of Bangladesh super sweet maize inbred linesxmaize inbred lines 12-9-10(DNA)was 2.91%,17h after pollination ,the result: 17h>19h>21h>23h. The highest mutant rate of DI generation from the combination of maize inbred lines 12-9-10 Bangladesh super sweet maize inbred lines (DNA) was 1.22% , the result: 19h>21h>17h> 23h.(2) Analysis of the number of chromosome: The number of chromosome of CKmongt , Ckwaxy and the plants Which were treated was added to or reduced .The reasons was studied further.(3) After the exogenous DNA from Bangladesh super sweet maize inbred lines introduced into the receptor of maize inbred lines 12-9-10,the mutant rate through pollen-tube pathway was higher than through seed-immersion method.