The Frist Stage Research Of The Exogenous DNA Pollen-tube Pathway Transferring In Lilium Davidii Var. Unicolor (hong) Cotton

Lilium davidii var.Unicolor(hong) is an variety of lily family with its characteristics as full-grown tender and white squamae; delicate texture; scent and bitterless taste;superior edible quality. Owing to its 400 years cultivation history in Lanzhou and its peripherical areas, the quality of some cultivated species of Lilium davidii var. Unicolor (hong) comes to retrogress. The Chinese research of lily's breeding can be fraced back in 1980's by using distant hybridization to get some lily's distant hytrids. Its breeding technique to improve lily's quality was stigma cutting and hybridized embryo cultivating. This thesis presets the literature review of lily's breeding and its genetic breeding transferring technique. In this thesis, it is stated that the technique of transferring the pollen-tube pathway to exogenous gene has available and bright prospect.In this experiment, the anthor uses Lilium davidii var.Unicolor(hong). Gansu wild lily and some decorative lily varieties as the different materials and makes the respectively systematic observation and concerned records on their traits, phenological periods.Through the hybridization, it is concluded that Lilium davidii var.Unicolor(hong) gretly differ from the wild lily varieties and decorative lily in traits; some of their traits can be mutually complementary, but it is difficult to hybridize them each other. It isn't subject to seeds and embryo forming under inter-hybridization condition. Their autogamy is also tested and the result show that most of lily varieties have low rata self-fruitfulness. Lilium davidii var. Unicolor(hong) in autogamy shows different the seed-setting percentage in different pollinating methods, with the result as the higher rate of self-fruitfulness through xenogamy. Based on hybridization, genetransferring through pollen-tube pathway to Lilium davidii van Unicolor(hong) is researched. The master of basic technique of exogenous DNA transferring to Lilium davidii var.Unicolor(hong) provides us a set of improved parameters based on the rata of fruit and embryo seeds.After pollinating the conclusion of the items of ovary enlargement, fruitfulness, embryo seeds of transferring exogenous DNA should be better transferred to Lilium davidii var.Unicolor(hong) after 3 hours of pollinating in that the rate of ovary enlargement and embryo forming in this period is respectively high.While, the form of transferring is also tested, which shows that the position and percentrite of DNA are of great effect on Lilium davidii var.Unicolor(hong). The drip DNA to plane of stigma and style or drip high concentration DNA will make the rate of ovary enlargement and embryo seeds decrease.In this experiment, 3 concentrations of DNA transferring solution is set up . The results show that the higher concentration DNA solution after pollinating will effect the embryo seeds. And it also show that when Lilium davidii var. Unicolor(hong) is accepting the exogenous DNA pollen-tube pathway transferring, the embryo seeds rate would be higher with 50 ug/ml concentration solution and the neutral drip DNA solution could be advisable.The gel electrophoresis of protein of the variation of the number of chromosome don't show any difference between transgene plant and their parental generation.